02603nas a2200205 4500008004100000245011400041210006900155260001400224300001200238490000600250520193200256653002602188653002502214653001802239653002102257653001802278100002002296700002302316856005802339 2017 eng d00aIn vitro Antidiabetic Activity of Polar and Nonpolar Solvent Extracts from Leucas aspera (Willd.) Link Leaves0 aIn vitro Antidiabetic Activity of Polar and Nonpolar Solvent Ext cJuly 2017 a261-2650 v93 a
Background: Diabetes mellitus is a chronic illness, and the management of diabetes is a global problem. Successful treatment is required to prevent complications and organ damages. Herbal medicines are having minimal adverse effects when compared to the available synthetic drugs to treat such chronic diseases and disorders. Objective: The present study was aimed to evaluate the antidiabetic and antioxidant activity of polar and nonpolar solvent extracts of Leucas aspera (Willd.) link leaves under in vitro models. Materials and Methods: The in vitro antidiabetic activity of petroleum ether (nonpolar) and ethanol (polar) extracts were evaluated in C2C12 cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (cell viability method) and glucose uptake assay. 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method used for the evaluation of in vitro antioxidant activity. Results: Both the polar and nonpolar solvent extracts of L. aspera had shown better antioxidant activity compared to standard (IC50 = 18.96 and 19.90 μg/mL, respectively). Petroleum ether extract exhibited better cytotoxic activity in C2C12 cell line compared to ethanol extract (concentration of test drug needed to inhibit cell growth by 50% 110.75 ± 5.5 vs. 415.25 ± 8.0 μg/mL) whereas ethanol extract showed enhanced glucose uptake activity than petroleum ether extract in C2C12 cell line at same concentrations. Conclusion: From our study results, we concluded that L. aspera (Willd.) link leaves had shown better antidiabetic activity and antioxidant activity under in vitro models. Nonpolar solvent extract produced slightly better activity than polar solvent extract. This study warrants further research and experiments on animal models.
10aIn vitro antidiabetic10aIn vitro antioxidant10aLeucas aspera10aNonpolar solvent10aPolar solvent1 aAnnapandian, VM1 aSundaram, Shanmuga uhttps://phcogres.com/article/2017/9/3/104103prpr14116