02898nas a2200325 4500008004100000245006900041210006900110260001400179300001200193490000600205520190400211653001102115653003802126653004202164653001402206653001802220653001402238653003102252653001702283653001802300653001902318100002802337700002702365700002202392700002502414700002402439700002102463700002402484856006402508 2015 eng d00aChemical Constituents and Bioactivities of Glinus Oppositifolius0 aChemical Constituents and Bioactivities of Glinus Oppositifolius cJune 2015 a138-1470 v73 a

Objectives: To isolate the secondary metabolites from the dichloromethane (DCM) extracts of Glinus oppositifolius; to test for the cytotoxicity of a new triterpene, oppositifolone (1); and to test for the hypoglycemic, analgesic, and antimicrobial potentials of 1, DCM and aqueous leaf extracts of G. oppositifolius. Methods: The compounds were isolated by silica gel chromatography and identified by nuclear magnetic resonance spectroscopy. The cytotoxicity potential of 1 was tested using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Triterpene 1, DCM, and aqueous leaf extracts were tested for hypoglycemic potential using the oral glucose tolerance test; analgesic potential using the tail‑flick assay, and antimicrobial potential using the disc diffusion method. Results: The DCM extracts of G. oppositifolius afforded 1, squalene, spinasterol, oleanolic acid, phytol, and lutein from the leaves; squalene and spergulagenin A from the stems; and spinasterol from the roots. Triterpene 1 was cytotoxic against human colon carcinoma 116 with an IC50 value of 28.7 but did not exhibit cytotoxicity against A549. The aqueous leaf extract at 200 mg/kg body weight (BW) exhibited hypoglycemic activity with a pronounced % blood glucose reduction of 70.76% ±17.4% within 0.5 h after introduction. The DCM leaf extract showed a lower % blood glucose reduction of 18.52% ±13.5% at 200 mg/kg BW within 1.5 h after introduction, while 1 did not exhibit hypoglycemic activity. The samples did not exhibit analgesic property and were inactive against multiple drug resistant bacterial pathogens. Conclusion: The compounds responsible for the hypoglycemic activity of G. oppositifolius which are fast acting (0.5 h) are found in the aqueous leaf extract.

10a3‑(410a5‑dimethylthiazol‑2‑yl)‑210a5‑diphenyltetrazolium bromide assay10aAnalgesic10aAntimicrobial10aCytotoxic10aGlinus oppositifolius Linn10aHypoglycemic10aMolluginaceae10aOppositifolone1 aRagasa, Consolacion, Y.1 aCabrera, Esperanza, C.1 aTorres, Oscar, B.1 aBuluran, Adiel, Inah1 aEspineli, Dinah, L.1 aRaga, Dennis, D.1 aShen, Chien‑Chang uhttps://phcogres.com/article/2015/7/2/1041030974-8490150520