02830nas a2200241 4500008004100000245012100041210006900162260001800231300001000249490000600259520203700265653000602302653003802308653002102346653001502367653001802382653002102400100001802421700003102439700002602470700002802496856006402524 2016 eng d00aIn vitro and In vivo Antioxidant Evaluation and Estimation of Total Phenolic, Flavonoidal Content of Mimosa pudica L0 aIn vitro and In vivo Antioxidant Evaluation and Estimation of To cDecember 2015 a22-280 v83 a

Objective: Mimosa pudica Linn. (Mimosaceae) is traditionally used as a folk medicine to treat various ailments including convulsions, alopecia, diarrhea, dysentery, insomnia, tumor, wound healing, snake bite, etc., Here, the study was aimed to evaluate the antioxidant potential of M. pudica leaves extract against 2, 2‑diphenyl‑1‑picrylhydrazyl (DPPH) (in vitro) and its modulatory effect on rat brain enzymes. Materials and Methods: Total phenolic, flavonoid contents, and in vitro antioxidant potential against DPPH radical were evaluated from various extracts of M. pudica leaves. In addition, ethyl acetate extract of Mimosa pudica leaves (EAMP) in doses of 100, 200, and 400 mg/kg/day were administered orally for 7 consecutive days to albino rats and evaluated for the oxidative stress markers as thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) from rat brain homogenate. Results: The ethyl acetate extract showed the highest total phenolic content and total flavonoid content among other extracts of M. pudica leaves. The percentage inhibition and IC50 value of all the extracts were followed dose‑dependency and found significant (P < 0.01) as compared to standard (ascorbic acid). The oxidative stress markers as SOD, CAT, and GSH were increased significantly (P < 0.01) at 200 and 400 mg/kg of EAMP treated animals and decreased significantly the TBARS level at 400 mg/kg of EAMP as compared to control group. Conclusion: These results revealed that the ethyl acetate extract of M. pudica exhibits both in vitro antioxidant activity against DPPH and in vivo antioxidant activity by modulating brain enzymes in the rat. This could be further correlated with its potential to neuroprotective activity due to the presence of flavonoids and phenolic contents in the extract.

10a210a2‑diphenyl‑1‑picrylhydrazyl10aBrain homogenate10aFlavonoids10aMimosa pudica10aOxidative stress1 aPatro, Ganesh1 aBhattamisra, Subrat, Kumar1 aMohanty, Bijay, Kumar1 aSahoo, Himanshu, Bhusan uhttps://phcogres.com/article/2016/8/1/1041030974-8490171099