02599nas a2200217 4500008004100000245020400041210007100245260001500316300001200331490000600343520175900349653004602108653002102154653001502175653006202190653001502252100001402267700001802281700001802299856006402317 2017 eng d00aRapid Development and Validation of Improved Reversed‑Phase High‑performance Liquid Chromatography Method for the Quantification of Mangiferin, a Polyphenol Xanthone Glycoside in Mangifera indica0 aRapid Development and Validation of Improved Reversed‑Phase High cApril 2017 a215-2190 v93 a
Mangiferin, a polyphenolic xanthone glycoside from Mangifera indica, is used as traditional medicine for the treatment of numerous diseases. The present study was aimed to develop and validate a reversed-phase high-performance liquid chromatography (RP-HPLC) method for the quantification of mangiferin from the bark extract of M. indica. RP-HPLC analysis was performed by isocratic elution with a low-pressure gradient using 0.1% formic acid: acetonitrile (87:13) as a mobile phase with a flow rate of 1.5 ml/min. The separation was done at 26°C using a Kinetex XB-C18 column as stationary phase and the detection wavelength at 256 nm. The proposed method was validated for linearity, precision, accuracy, limit of detection, limit of quantification, and robustness by the International Conference on Harmonisation guidelines. In linearity, the excellent correlation coefficient more than 0.999 indicated good fitting of the curve and also good linearity. The intra- and inter-day precision showed <1% of relative standard deviation of peak area indicated high reliability and reproducibility of the method. The recovery values at three different levels (50%, 100%, and 150%) of spiked samples were found to be 100.47, 100.89, and 100.99, respectively, and low standard deviation value <1% shows high accuracy of the method. In robustness, the results remain unaffected by small variation in the analytical parameters, which shows the robustness of the method. Liquid chromatography–mass spectrometry analysis confirmed the presence of mangiferin with M/Z value of 421. The assay developed by HPLC method is a simple, rapid, and reliable for the determination of mangiferin from M. indica.
10aLiquid chromatography–mass spectrometry10aMangifera indica10aMangiferin10aReversed‑phase high‑performance liquid chromatography10aValidation1 aNaveen, P1 aLingaraju, HB1 aPrasad, Shyam uhttps://phcogres.com/article/2017/9/2/1041030974-8490204652