02269nas a2200217 4500008004100000245013000041210006900171260001700240300001200257490000700269520154200276653002401818653001901842653002301861653001601884653001701900100003601917700001601953700002401969856005801993 2018 eng d00aThe Effect of Capsicum Oleoresin on Nitric Oxide Production and Nitric Oxide Synthase Gene Expression in Macrophage Cell Line0 aEffect of Capsicum Oleoresin on Nitric Oxide Production and Nitr cOctober 2018 a343-3460 v103 a

Background: Nitric oxide (NO) is an inflammatory agent produced by macrophages. It also acts as a neurotransmitter. However, overproduction of NO results in acute or chronic inflammation. Capsicum is well known for its anti‑oxidant, anti–inflammatory, and anticancer properties. Objective: The objective of this study was to evaluate the effect of capsicum oleoresin on NO production and NO synthase gene expression in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophage cell line. Materials and Methods: Macrophage RAW 264.7 cells were obtained from the National Centre for Cell Science, Pune with Passage no 16. RAW macrophages were treated with 12.5 μg/ml, 25 μg/ml, and 50 μg/ml of Capsicum extract with 1 μg/ml of LPS and incubated for 24 h. Results: When capsicum was added at three different doses of 12.5 μg/ml, 25 μg/ml, and 50 μg/ml, the inducible NO synthase (iNOS) levels was significantly suppressed, compared to that of LPS treatment only. The level of NO increased by LPS induction was significantly decreased in a dose‑dependent manner when treated with different concentrations of capsicum extract and capsicum had a suppressing effect on iNOS gene expression in LPS – stimulated RAW 264.7 macrophage. Conclusion: This study concludes that capsicum oleoresin is good enough to suppress iNOS gene expression and NO production. Hence, it may be used in inflammatory conditions with excessive NO production.

10aAnti‑inflammatory10aAnti‑oxidant10aCapsicum oleoresin10aMacrophages10aNitric oxide1 aPrathoshni, Srinivasan, Manthra1 aAnitha, Roy1 aLakshmi, Thangavelu uhttps://phcogres.com/article/2018/10/4/104103prpr4618