02228nas a2200229 4500008004100000245011200041210006900153260001500222300001200237490000700249520147000256653001701726653001701743653001501760653002301775100002301798700003001821700002301851700002901874700003601903856005901939 2018 eng d00aAntioxidant and Cytotoxic Potential of Endophytic Fungi Isolated from Medicinal Plant Tragia involucrata L.0 aAntioxidant and Cytotoxic Potential of Endophytic Fungi Isolated cApril 2018 a188-1940 v103 a

Objective: The aim of the present study was to test the antioxidant and cytotoxic and DNA protection activities of ethyl acetate extracts of endophytic fungi isolated from Tragia involucrata Linn. (Euphorbiaceae). Materials and Methods: The 1, 1‑diphenyl‑2‑picrylhydrazyl scavenging, reducing power, and total antioxidant assay were used to evaluate the antioxidant activity. Cytotoxic activity of endophytic fungal extracts against MCF‑7 and MOLT‑4 cell lines was carried out using 3‑(4, 5‑dimethylthiazol‑2‑yl)‑2, 5‑diphenyl‑2H‑tetrazolium bromide method. Results: We have successfully isolated four endophytic fungi from T. involucrata, namely Penicillium citrinum CGJ‑C1 (GenBank accession No. KT780618), P. citrinum CGJ‑C2 (GenBank accession No. KP739821), Cladosporium sp. CGJ‑D1 (GenBank accession No. KP739822), and Cryptendoxyla hypophloia CGJ‑D2 (GenBank accession No. KT780619). The ethyl acetate extract of P. citrinum CGJ‑C2 showed the highest antioxidant as well as cytotoxic activity among the four fungal extracts taken for the study. All the extracts showed moderate DNA protection activity. Conclusions: Further studies on the isolation and purification of the lead molecule will help in designing the novel therapy for different ailments associated with free radical generation.

10aAntioxidants10acytotoxicity10aEndophytes10aTragia involucrata1 aDanagoudar, Ananda1 aJoshi, Chandrashekhar, G.1 aRavi, Sunil, Kumar1 aKumar, Heggodu, G. Rohit1 aRamesh, Balenahalli, Narasingap uhttps://phcogres.com/article/2018/10/2/104103prpr13717