02454nas a2200277 4500008004100000245011300041210006900154260001800223300001000241490000600251520153800257653003001795653004601825653003401871653003301905100001601938700001801954700001801972700002301990700002002013700001802033700001802051700002302069700002002092856006402112 2017 eng d00aGrowth Inhibition and Morphological Alteration of Fusarium sporotrichioides by Mentha piperita Essential Oil0 aGrowth Inhibition and Morphological Alteration of Fusarium sporo cFebruary 2017 a74-790 v93 a

Objective: The aim of this study is to determine the phytochemical composition, antifungal activity of Mentha piperita essential oil (MPE) against Fusarium sporotrichioides. Methods: The phytochemical composition was conducted by gas chromatography mass spectrometry (GC MS) analysis and mycelia growth inhibition was determined by minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC), the morphological characterization was observed by scanning electron microscopy. Finally, the membrane permeability was determined by the release of extracellular constituents, pH, and total lipid content. Result: In GC MS analysis, 22 metabolites were identified such as menthol, l menthone, pulegone, piperitone, caryophyllene, menthol acetate, etc. The antifungal activity against targeted pathogen, with MIC and MFC 500 μg/mL and 1000 μg/mL, respectively. The MPE altered the morphology of F. sporotrichoides hyphae with the loss of cytoplasm content and contorted the mycelia. The increasing concentration of MPE showed increase in membrane permeability of F. sporotrichoides as evidenced by the release of extracellular constituents and pH with the disruption of cell membrane indicating decrease in lipid content of F. sporotrichoides. Conclusion: The observed results showed that MPE exhibited promising new antifungal agent against Fusarium sporotrichioides

10aFusarium sporotrichioides10aGas chromatography-mass spectrometryGC-MS10aMentha piperita essential oil10aScanning electron microscope1 aRachitha, P1 aKrupashree, K1 aJayashree, GV1 aGopalan, Natarajan1 aKhanum, Farhath1 aKrupashree, K1 aJayashree, GV1 aGopalan, Natarajan1 aKhanum, Farhath uhttps://phcogres.com/article/2017/9/1/1041030974-8490199771