03072nas a2200289 4500008004100000245011500041210006900156260001800225300000800243490000700251520207900258653002702337653002502364653002102389653002302410653002902433100003002462700002702492700003102519700002902550700002702579700002702606700003302633700002702666700002802693856006102721 2019 eng d00aChemical Characterization and Evaluation of Antioxidant and Antimicrobial Activities of Litchi chinensis Sonn.0 aChemical Characterization and Evaluation of Antioxidant and Anti cFebruary,2019 a1-70 v113 a

Background: Litchi chinensis is used in traditional Chinese medicine and by Indian medical system. Ethnopharmacological studies show anti‑inflammatory, antidiabetic and analgesic activities, among others. However, there are few studies of antimicrobial activity. This study evaluates antimicrobial, antioxidant, and cytotoxicity properties of the lychee’s leaves extract (LE) and fractions. Materials and Methods: Extracts were obtained using an exhaustive extraction method with ethanol: Water (7:3 v/v). Subsequently, LE was concentrated in a rotary evaporator. Finally, LE was dried via lyophilization. Fractions were obtained via the partition process. Bioactivity of the LE and fractions (hexane [Hex], ethyl acetate [EtOAc], n‑butanol [BuOH], and aqueous [Aq]) from L. chinensis was evaluated through antimicrobial activity using broth microdilution, antioxidant activity via both 1,1‑diphenyl‑2‑picryl‑hidrazila assay and ferric reducing capacity and cytotoxicity through 3‑(4,5‑dimethyl‑thiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide reduction assay. Furthermore, mass spectrometry technique electrospray ionization ion trap mass spectrometry was used to identify the chemical composition of the LE and fractions. Results: Phenolic compounds, such as flavonoids and condensed tannins were the main substances found. Total phenolic and flavonoid contents were higher in EtOAc (541.15 ± 2.4 mg/g and 31.06 ± 0.5 mg/g, respectively). This fraction showed the best results for antioxidant activity (IC50 = 3.45 mg/mL) and ferric reducing capacity (20.27% ± 0.11). The LE and fractions showed considerable antimicrobial activity, chiefly against Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, and Proteus mirabilis, with the minimum inhibitory concentration ranging from 50 to 1560 μg/ml. Conclusion: This study revealed that L. chinensis is a source of bioactive compounds potentially useful for pharmaceutical and food industries.

10aAntimicrobial activity10aAntioxidant activity10aMedicinal plants10aPhenolic compounds10aPhytochemical screening.1 aLopes, Marisa, De Oliveir1 aDa Silva, Ana, Flávia1 aCerdeira, Cláudio, Daniel1 aRibeiro, Ingridy, Simone1 aRosa, Isael, Aparecido1 aReis, Luis, Felipe Cun1 aDa Silva, Marcelo, Aparecido1 aMarques, Marcos, José1 aChavasco, Jorge, Kleber uhttps://phcogres.com/article/2019/11/1/104103prpr10518-0