02329nas a2200253 4500008004100000245020100041210006900242260001800311300001000329490000700339520141200346653001301758653001701771653001501788653002301803653005701826653001501883100003101898700001701929700003201946700002001978700001901998856005802017 2018 eng d00aAnalytical Method Development and Validation for the Simultaneous Estimation of Abacavir and Lamivudine by Reversed‑phase High‑performance Liquid Chromatography in Bulk and Tablet Dosage Forms0 aAnalytical Method Development and Validation for the Simultaneou cFebruary 2018 a92-970 v103 a

Objective: A simple rapid, accurate, precise, and reproducible validated reverse phase high performance liquid chromatography (HPLC) method was developed for the determination of Abacavir (ABAC) and Lamivudine (LAMI) in bulk and tablet dosage forms. Methods: The quantification was carried out using Symmetry Premsil C18 (250 mm × 4.6 mm, 5 μm) column run in isocratic way using mobile phase comprising methanol: water (0.05% orthophosphoric acid with pH 3) 83:17 v/v and a detection wavelength of 245 nm and injection volume of 20 μl, with a flow rate of 1 ml/min. Results: In the developed method, the retention times of ABAC and LAMI were found to be 3.5 min and 7.4 min, respectively. The method was validated in terms of linearity, precision, accuracy, limits of detection, limits of quantitation, and robustness in accordance with the International Conference on Harmonization guidelines. Conclusion: The assay of the proposed method was found to be 99% – 101%. The recovery studies were also carried out and mean % recovery was found to be 99% – 101%. The % relative standard deviation from reproducibility was found to be <2%. The proposed method was statistically evaluated and can be applied for routine quality control analysis of ABAC and LAMI in bulk and in tablet dosage form.

10aAbacavir10aDosage forms10aLamivudine10aMethod development10aReverse phase high performance liquid chromatography10aValidation1 aAhmad, Sufiyan, Ahmad Raee1 aPatil, Lalit1 aUsman, Mohammed, Rageeb Moh1 aImran, Mohammad1 aAkhtar, Rashid uhttps://phcogres.com/article/2018/10/1/104103prpr9617