02225nas a2200253 4500008004100000245012200041210006900163260001800232300001000250490000700260520138000267653000601647653004901653653001601702653003101718653002001749100001801769700002801787700002201815700002201837700002801859700002601887856005801913 2018 eng d00aIn vitro Evaluation of Antioxidant Potential of Isolated Compounds and Various Extracts of Peel of Punica granatum L.0 aIn vitro Evaluation of Antioxidant Potential of Isolated Compoun cFebruary 2018 a44-480 v103 a

Background: Punica granatum L. (Lythraceae) peel has been proven to exhibit widespread pharmacological application against multitude of diseases due to the presence of bioactive principles. Objective: The objective is to isolate the bioactive compounds from the pericarp of P. granatum and to evaluate the antioxidant activity of various extracts. Materials and Methods: Dried peel of P. granatum was extracted with aqueous acetone and chromatographed on Diaion HP‑20. Enriched fractions were rechromatographed on Sephadex LH‑20 and purified on preparative high‑performance liquid chromatography to identify individual compounds. The dried peel was extracted with different solvents to evaluate the antioxidant activity of the extracts. Results: On the chemical investigation, three compounds were isolated and characterized as punicalagin, 2,3‑(S)‑hexahydroxydiphenoyl‑D‑glucose, and punicalin, using various spectroscopic techniques. Conclusion: Results indicate that the isolated compounds have possessed antioxidant activity, and aqueous, methanol, and aqueous acetone extract showed significant scavenging of 2,2‑diphenyl‑1‑picrylhydrazyl and 2,2’‑azino‑bis (3‑ethylbenzthiazoline‑6‑sulfonic acid) radicals.

10a210a3‑(S)‑hexahydroxydiphenoyl‑D‑glucose10aAntioxidant10aNuclear magnetic resonance10aPunica granatum1 aJacob, Janani1 aLakshmanapermalsamy, P.1 aIlluri, Ramanaiah1 aIlluri, Ramanaiah1 aSangli, Gopala, Krishna1 aMundkinajeddu, Deepak uhttps://phcogres.com/article/2018/10/1/104103prpr3617