02712nas a2200277 4500008004100000245016700041210006900208260001700277300001200294490000700306520170400313653002702017653001402044653001802058653003702076653001702113100003502130700002702165700002802192700003402220700003202254700003002286700002602316700003402342856005802376 2020 eng d00aAnatomical Description and in vitro Evaluation of the Antibacterial Potential of Aristolochia esperanzae Kuntze (Aristolochiaceae) Extract on Oral Micro-organisms0 aAnatomical Description and in vitro Evaluation of the Antibacter cJanuary 2021 a424-4290 v123 a

Background: Aristolochia esperanzae Kuntze (Aristolochiaceae) is a plant present in the Brazilian flora, with few studies in the dental area, but used in integrative therapies to treat some health problems. Objectives: The initial purpose was to make an anatomical description of A. esperanzae and then test its antibacterial activity and minimum inhibitory concentration (MIC) on specific oral micro-organisms‑Streptococcus mutans, Porphyromonas gingivalis and Enterococcus faecalis. Materials and Methods: For the pharmacognostic study, the samples were fixed in formaldehyde, acetic acid and 70% ethyl alcohol and stored in 70% ethanol. For the in vitro study, to obtain the essential oil (OE) and hydrolate, the plant material was mechanically macerated and subjected to hydrodistillation in Clevenger and then frozen until the time of testing. The EO was diluted in dimethylsulfoxide and the tests performed were agar diffusion (disc‑diffusion) with isolated bacteria and MIC. A 0.12% chlorhexidine digluconate solution was used as a positive control and 0.9% sodium chloride as a negative. Results: The anatomical study showed the presence of hook‑shaped trichomes and stomatal complexes. Regarding the microbiological results, they were negative for the extracts used. Conclusion: The anatomical findings may guide future studies related to the pharmacognostic properties of A. esperanzae and both OE and hydrolate did not have an antibacterial effect against S. mutans, P. gingivalis and E. faecalis.

10aAntimicrobial activity10aDentistry10aEssential oil10aMinimum inhibitory concentration10aPhytotherapy1 aBotelho-Filho, Carlos, Roberto1 aIzumi, Gabriela, Keiko1 aVieira, Juliana, Campos1 aBertoli, Fernanda, Mara de Pa1 aFilho, Antônio, Pedro Rico1 aMaranho, Leila, Teresinha1 aBaratto-Filho, Flares1 aGabardo, Marilisa, Carneiro L uhttps://phcogres.com/article/2020/12/4/104103prpr5220