02989nas a2200241 4500008004100000245012300041210006900164260001800233300000800251490000700259520217300266653002602439653002502465653003002490653002802520653003302548653002102581100001702602700002302619700001702642700002602659856006202685 2026 eng d00aPhytochemical Profiling and Standardization of Punarnavastak Kwath Ghanvati Using Physicochemical Parameters and HPTLC0 aPhytochemical Profiling and Standardization of Punarnavastak Kwa cNovember 2025 a1-70 v183 a

Background: Punarnavastak Kwath, a classical Ayurvedic decoction described in the Bhaishajya Ratnavali, is renowned for its efficacy in managing Udara Roga (abdominal disorders) and Shotha Roga (oedema). To enhance patient compliance, this formulation is transformed into a concentrated tablet known as Punarnavastak Kwath Ghanvati (PAKG), prepared without any excipients. Despite extensive traditional use, scientific validation of PAKG through contemporary analytical techniques remains necessary to ensure consistent therapeutic effectiveness and quality control. Purpose: The present study aimed to conduct a comprehensive evaluation of PAKG through organoleptic examination, physico-chemical analysis, phytochemical screening, and High-Performance Thin-Layer Chromatography (HPTLC). Materials and Methods: The formulation was prepared as per the guidelines stipulated in the Indian Pharmacopoeia. Organoleptic characteristics (colour, taste, odour), along with moisture content, total ash, acid-insoluble ash, water-soluble ash, and extractive values (aqueous and alcoholic), were systematically assessed. Preliminary phytochemical screening was conducted to detect major bioactive groups such as alkaloids, flavonoids, tannins, saponins, and glycosides. Furthermore, HPTLC profiling was performed to identify and quantify specific phytochemical constituents, documenting characteristic retention factor (Rf) values essential for establishing reproducible quality standards. Results: The analysis revealed consistent physico-chemical parameters indicative of formulation stability and quality. Phytochemical screening confirmed the presence of active constituents, reinforcing traditional claims. HPTLC analysis provided reproducible fingerprints, enabling precise standardization of PAKG. Conclusion: This integrative scientific evaluation effectively bridges traditional Ayurvedic knowledge and contemporary analytical validation, ensuring therapeutic consistency, enhancing credibility, and facilitating wider acceptance of Punarnavastak Kwath Ghanvati in modern healthcare practice.

10aAyurvedic formulation10aHPTLC fingerprinting10aPhysico-chemical analysis10aPhytochemical screening10aPunarnavastak Kwath Ghanvati10aStandardization.1 aDonga, Dixit1 aToshikhane, Hemant1 aDaga, Harish1 aGohil, Dhairyjeetsinh uhttps://phcogres.com/article/2026/18/1/105530pres20260057