02655nas a2200277 4500008004100000245016000041210007100201260001900272300001400291490000700305520175800312653001002070653001802080653003302098653001502131653001902146100001802165700001602183700001602199700001902215700001502234700002302249700002502272700001802297856006202315 2025 eng d00aBox–Behnken Design Based Optimisation of HPTLC Method for Analysis of Jatrorrhizine from Tinospora cordifolia Roots with Antidiabetic Activity Assessment0 aBox–Behnken Design Based Optimisation of HPTLC Method for Analys cSeptember 2025 a1382-13940 v173 a

Introduction: Tinospora cordifolia root (T. cordifolia) is a well-known Ayurvedic herb with immune-boosting, anti-inflammatory, and antioxidant properties. It contains Jatrorrhizine (JZ), a bioactive alkaloid with antimicrobial, hepatoprotective, and metabolic health benefits, similar to berberine. Objectives: The study aimed to evaluate the JZ content in T. cordifolia root obtained via different extraction method and to optimise the High-Performance Thin Layer Chromatography (HPTLC) method via Response Surface Methodology (RSM). The extracts were assessed for in vitro test evaluation. Materials and Methods: A Box-Behnken Design (BBD) of RSM, a mathematical and statistical method, was applied to optimize the impact of independent variable on the response. Three independent variables like the different concentration of the mobile phase were studied. The in vitro analysis was also done. Results: The content of JZ was quantified using the HPTLC densiometric method. HPTLC confirmed the presence of JZ with a prominent band at an Rf value of 0.70±0.008 using a mobile phase of chloroform: toluene: methanol: formic acid (7:4:2:0.2 v/v). The in vitro analysis of the extract showed a significant result and was found to be α-amylase in TRM 27.71±0.70 and α-glucosidase in TRM 43.45±1.31. Conclusion: This work examines various extraction procedures, including Soxhlet, maceration and reflux, and introduces a novel methodology to enhance extraction efficiency, advance the phytochemical analysis of JZ. This suggests that T. cordifolia may have therapeutic potential in diabetes management.

10aHPTLC10aJatrorrhizine10aResponse surface methodology10aα-Amylase10aα-Glucosidase1 aKumar, Neeraj1 aA, Akanksha1 aTyagi, Rama1 aSingh, Shubham1 aAqil, Mohd1 aNajmi, Abul, kalam1 aIsmail, Mohd, Vaseem1 aMujeeb, Mohd. uhttps://phcogres.com/article/2025/17/4/105530pres20252320