02016nas a2200217 4500008004100000245012500041210006900166260001600235300001400251490000700265520128900272653001601561653001901577653001801596653001401614100001701628700003001645700003401675700002701709856006201736 2025 eng d00aIsolation of Bioactive Metabolites from Tephrosia maxima and Tephrosia callophylla: A Phytochemical and Functional Study0 aIsolation of Bioactive Metabolites from Tephrosia maxima and Tep cAugust 2025 a1341-13470 v173 a

Tephrosia maxima and Tephrosia callophylla, two lesser-studied members of the Fabaceae family, were evaluated for their phytochemical, pharmacological, and toxicological profiles in this study. Aqueous extracts from shade-dried leaves were subjected to SEM analysis, hemocompatibility testing, and assays for anti-bacterial, anti-cancer, anti-inflammatory, antioxidant, and general toxicity. SEM revealed irregular crystalline morphologies, indicative of diverse bioactive constituents. Both extracts exhibited <2% hemolysis, confirming systemic biocompatibility. Antibacterial activity against E. coli and S. aureus was evident, along with potent cytotoxicity against MCF-7 breast cancer cells. Anti-inflammatory and antioxidant assays demonstrated dose-dependent efficacy, with up to 80% nitric oxide inhibition and 90% DPPH radical scavenging. Toxicity studies using brine shrimp and zebrafish embryos showed high viability and minimal adverse effects. These findings highlight the therapeutic promise and biosafety of T. maxima and T. callophylla, supporting their potential for drug discovery and biomedical applications. Further studies are needed to isolate active compounds and elucidate molecular mechanisms.

10aAnti-cancer10aAnti-microbial10aPhotochemical10aTephrosia1 aKumar, Anoop1 aRamasubramanian, Abilasha1 aMuthumanickam, Priyadharshini1 aPurushothaman, Bargavi uhttps://phcogres.com/article/2025/17/4/105530pres20250024