02319nas a2200241 4500008004100000245019500041210006900236260001400305300001200319490000700331520141400338653002501752653002101777653001901798653002701817100002801844700003601872700002401908700002801932700002001960700003501980856006202015 2025 eng d00aPhytochemical Profiling, Antioxidant and Antimicrobial Activities of a Traditional Polyherbal Formulation Containing Citrus limon, Bergera koenigii, Citrus medica and Andrographis paniculata0 aPhytochemical Profiling Antioxidant and Antimicrobial Activities cJune 2025 a881-8950 v173 a

Background: Polyherbal formulations combining medicinal plants are widely studied for their enhanced therapeutic potential. Materials and Methods: This study evaluates a polyherbal formulation containing leaves of Citrus limon, Bergera koenigii, Citrus medica, and Andrographis paniculata for its antimicrobic and antioxidative properties. An ethanolic extract was examined to determine its phytochemical composition, which revealed a rich existence of bioactive substances like bioflavonoids, alkali-like compounds, phenolics, and Steroidal glycoalkaloids. Results: In vitro Total Antioxidant Capacity (TAC), including DPPH radical, FRAP and scavenging of nitric oxide, exhibited significant radical-neutralizing capacities comparable to standard antioxidants. The formulation also exhibited potent antibacterial action against strains of fungi and bacteria, both gram-positive and gram-negative, suggesting a synergistic enhancement of efficacy due to the combination of plant extracts. Conclusion: The outcomes highlight the prospect of this polyherbal extract as a natural therapeutic agent to treat microbial infections and oxidative stress. The future studies should focus upon in vivo evaluations and elucidating molecular mechanisms to validate these promising results.

10aNatural therapeutics10aOxidative stress10aPhytochemicals10aPolyherbal formulation1 aGnanadeepan, Muthuswamy1 aSakthivel, Kunnathur, Murugesan1 aRasmi, Rajan, Radha1 aThirumalai, Venkatraman1 aPavithra, Kumar1 aNachimuthu, Kannikaparameswari uhttps://phcogres.com/article/2025/17/3/105530pres20252131