02729nas a2200253 4500008004100000245010000041210006900141260001500210300000900225490000700234520195300241653002002194653001502214653001902229653001802248653002002266100001702286700002602303700001802329700001802347700002002365700002802385856006202413 2025 eng d00aStandardization and GC-MS Analysis of Phytochemicals in Coccinia indica (Wight and Arn.) Fruits0 aStandardization and GCMS Analysis of Phytochemicals in Coccinia  cApril 2025 a1-120 v173 a

Background: Coccinia indica, commonly known as the ivy gourd, is traditionally utilized for its diverse medicinal properties. Objectives: The objectives of the study were to standardize and evaluate the fruits of Coccinia indica by identifying the phytochemicals using GC-MS analysis. Materials and Methods: Standard methodologies were used for the standardization, including macroscopical, microscopical, physico-chemical evaluations and phytochemical screening. Additionally, GC-MS analysis was performed to identify key components in the plant extract and its fractions. Results: The T.S. of the fruits of C. indica showed a single epicarp, mesocarp and several fibro-vascular bundles, while the T.S. of the seed revealed the presence of a testa, tegmen, embryo, starch grains and several oil globules. The main findings were as follows: total ash 16.52±0.13% w/w, water-soluble ash 11.35±0.12% w/w, acid-insoluble ash 1.45±0.06% w/w, water-soluble extractive value 18.45±0.2% w/w, alcohol-soluble extractive value 8.80±0.11% w/w, moisture content 7.33±0.08% w/w and the pH of the aqueous extract was 5.63±0.07 (1% w/v) and 5.79±0.09 (10% w/v). Phytochemical investigation revealed the presence of alkaloids, steroids, flavonoids, saponins, tannins, phenolics and terpenoids. GC-MS analysis identified more than forty notable chemicals in the methanol extract and fractions of chloroform and hexane. Conclusion: This study provides key insights into the standardization of C. indica fruits and identifies 43 major secondary metabolites through GC-MS analysis. Notable compounds include taraxerone, squalene, lupeol and piperine, all with potential therapeutic benefits. Moreover, the choice of solvent for extraction and fractionation significantly influenced the phytochemical profile and concentrations.

10aCoccinia indica10aEvaluation10aGC-MS Analysis10aPhytochemical10aStandardization1 aAlam, Shadab1 aKhan, Mohammad, Irfan1 aB, Badruddeen1 aAkhtar, Juber1 aAhmad, Mohammad1 aAhmad, Mohammad, Dabeer uhttps://phcogres.com/article/2025/17/2/105530pres20251942