02211nas a2200205 4500008004100000245012200041210006900163260001700232300001200249490000700261520154400268653002001812653001501832653001001847653001901857653001501876100002801891700002601919856006001945 2024 eng d00aHigh-Performance Thin Layer Chromatography (HPTLC) Analysis for the Quantification of Constituents in Ficus religiosa0 aHighPerformance Thin Layer Chromatography HPTLC Analysis for the cOctober 2024 a929-9340 v163 a

Background: Ficus religiosa, revered for its medicinal properties in traditional medicine, has drawn scientific interest due to its diverse chemical composition. This study aimed to develop a densitometric High-Performance Thin Layer Chromatography (HPTLC) technique to estimate phenolic compounds present in the hydroalcoholic extract of dried root bark of Ficus religiosa. Materials and Methods: This study utilized HPTLC to identify the constituents within Ficus religiosa root bark. The isolated constituent was evaluated by various analytical methods like phytochemical tests, UV, HPTLC etc. Subsequently, the HPTLC method underwent rigorous validation, confirming its reliability and accuracy in separating and quantifying the constituents within the root bark of Ficus religiosa. Results: Isolation of phytoconstituents was done by column chromatographic technique using toluene: ethyl acetate: methanol (3:3:2). The isolated component was confirmed as a flavonoid moiety by various chemical tests and by analytical evaluation. Also, HPTLC analysis of the isolate revealed the compound was Quercetin a flavonoid moiety or its derivative. Conclusion: These findings underscore the presence of main phytoconstituents in Ficus religiosa root bark which is responsible for pharmacological effects and offers insights into its application in both traditional and contemporary medicinal practices.

10aFicus religiosa10aFlavonoids10aHPTLC10aQuantification10aValidation1 aGangadharan, Jyothisree1 aSankararajan, Umadevi uhttps://phcogres.com/article/2024/16/4/105530pres164106