02306nas a2200265 4500008004100000245013600041210006900177260001500246300001000261490000700271520139300278653001401671653002701685653001901712653002501731653000901756100003301765700002601798700003301824700003101857700003301888700002901921700003201950856005801982 2021 eng d00aAntiarthritic and Antinociceptive Potential of Ethanolic Extract from Leaves of Doliocarpus dentatus (Aubl.) Standl. in Mouse Model0 aAntiarthritic and Antinociceptive Potential of Ethanolic Extract cApril 2021 a28-330 v133 a

Objectives: The folk use of Doliocarpus dentatus for pain and inflammatory conditions led our group to evaluate the anti-inflammatory, antinociceptive, and antiarthritic effects of its ethanol extract from the leaves (EEDd) on mouse models. Results: Oral treatments with EEDd (100–300 mg/kg) significantly inhibited the formalin-induced nociceptive and cold sensitivity, prevented acetic acid-induced nociceptive behavior, and prevented articular inflammation (including knee edema, leukocyte infiltration, and mechanical hyperalgesia) induced by zymosan. In the peritonitis model, betulinic acid (BA, 0.3–30 mg/kg) and EEDd (300 mg/kg) significantly inhibited zymosan-induced leukocyte infiltration. In the complete Freund adjuvant (CFA) model, oral treatments with EEDd (100–300 mg/kg) for 21 days significantly inhibited mechanical hyperalgesia, cold response, and edema. In the MTT viability assay, EEDd (3–90 μg/mL) did not induce leukocytes cytotoxicity. cytotoxicity. Most models employed male and female Swiss mice or, for the CFA test, C57BL/6 mice. Conclusion: This study demonstrates that EEDd exhibited antinociceptive, antihyperalgesic, and antiarthritic potential in mice and BA contribute for the EEDd observed activities.

10aArthritis10aArticular inflammation10aBetulinic acid10aDoliocarpus dentatus10aPain1 aBranquinho, Lidiane, Schultz1 aVerdan, Maria, Helena1 aSilva-Filho, Saulo, Euclides1 aOliveira, Rodrigo, Juliano1 aCardoso, Claudia, Andrea Lim1 aArena, Arielle, Cristina1 aKassuya, Candida, Aparecida uhttps://phcogres.com/article/2021/13/1/104103prpr7920