02258nas a2200253 4500008004100000024000700041245017100048210006900219260001800288300001200306490000700318520139800325653002401723653001501747653002001762653001701782653001701799653001401816100003001830700002801860700002901888700002801917856005901945 2023 eng d a2200aEvaluation of the Effect of Five Colombian Propolis Extracts on the Expression of Genes Associated with Cell Cycle and Inflammation in a Canine Osteosarcoma Cell Line0 aEvaluation of the Effect of Five Colombian Propolis Extracts on  cDecember 2023 a172-1820 v163 a

Introduction: Propolis has anti-inflammatory, antitumor, antibacterial and immunomodulatory properties, which is why it is suggested that it could be used as an alternative or complementary drug therapy in the treatment of various pathologies such as cancer, diseases chronic-degenerative and infectious. Materials and Methods: In this study, canine bone fibroblasts were used as control cells, and the canine Osteosarcoma cell line (OSCA-8) was acquired to evaluate the effects of ethanol extracts of propolis from five Colombian regions on these cells. The cytotoxic effect was evaluated by examining changes in cell viability and proliferation. Furthermore, the expression of some relevant and characteristic genes related to the tumor phenotype, related to the proinflammatory process and cell cycle, was assessed. Results: Thus, the evaluation of the relative expression of some genes associated with the cell cycle and inflammation could improve the understanding of the cytotoxic effect of propolis extracts on OSCA-8 cell lines. Conclusion: The first time in Colombia, the biological activity of ethanol extracts of propolis was evaluated regarding the inflammation and cell cycle pathways. After 48 hr, the Colombian EEP had an effect on the increase in both OSCA-8 cells and fibroblasts.

10aBiological activity10aCell cycle10aGene expression10aInflammation10aOsteosarcoma10aPropolis.1 aTorres, Oscar, Julián Mu1 aMora, Dolly, Patricia P1 aGarcía, Orlando, Torres1 aBuitrago, Mauricio, Rey uhttps://phcogres.com/article/2023/16/1/105530pres16122