02137nas a2200253 4500008004100000024000700041245014900048210006900197260001800266300001200284490000700296520130300303653003001606653000901636653002801645653001401673100002901687700002201716700001901738700002201757700002201779700002201801856006001823 2023 eng d a2100aSeparation of Methanolic Leaf Extracts of Three Rutaceous Plants by Capillary Electrophoresis and High-Performance Liquid Chromatography Methods0 aSeparation of Methanolic Leaf Extracts of Three Rutaceous Plants cFebruary 2023 a392-3980 v153 a

Introduction: In the phytochemical analysis of various multi component mixtures, Capillary electrophoresis (CE) method was considered as the best alternative tool due to high efficiency resolution separations, low solvent consumption and less maintenance costs. Materials and Methods: This study uses CE and high-performance liquid chromatography (HPLC) methods to evaluate the major components of methanolic leaf extract of Rutaceous plants. And also, to assess the purity of the components in isolated fractions and most importantly for the qualitative identification of the individual components of extracts based upon the relative retention time and UV profile. Results: The separation of the compounds in HPLC was attained by using ACE-5-C18 (250 x 4.6 mm) with a flow rate of 1.5 ml/min, with the UV detection at 254 nm and CE was equipped with a UV diode-array detector and bare, fused silica capillaries (56 cm 50 μm) with micro extended light path were used for the separations. Conclusion: Our study reveals that CE technique is a simple, economical and convenient method and shown high-resolution for multi-components found in plant extracts compared with HPLC profiles.

10aCapillary Electrophoresis10aHPLC10aRelative Retention Time10aRutaceae.1 aGuntupalli, Chakravarthi1 aMalothu, Narender1 aAreti, Ankarao1 aNalluri, Buchi, N1 aSivadasu, Praveen1 aAlla, Narayanarao uhttps://phcogres.com/article/2023/15/2/105530pres152042