02846nas a2200277 4500008004100000024000700041245010400048210006900152260001800221300001200239490000700251520193700258653002202195653002402217653001702241653002602258653001102284100002902295700003002324700003702354700002602391700003702417700002602454700002802480856006002508 2023 eng d a1300aAntidiabetic Potential and Chronic Toxicity of Hydroalcoholic Extract of Echeveria subrigida Leaves0 aAntidiabetic Potential and Chronic Toxicity of Hydroalcoholic Ex cFebruary 2023 a315-3270 v153 a

Background: Echeveria subrigida extracts have biological activities of human health importance. However, the in vivo effects on glucose levels and chronic toxicity are unknown. Objectives: To analyse the in vivo hypoglycemic and antihyperglycemic effects (50, 100, and 200 mg/kg b.w.) and chronic toxicity (1000 mg/kg b.w. for 270 days) of the hydroalcoholic extract of E. subrigida (HE–Es) in BALB/c mice. Materials and Methods: The HE–Es was analysed by HPLC. Glucose levels were measured to establish the effects on glycemia. Different parameters were registered in the toxicity assay: e.g., feed consumption, appearance/behaviour, biochemical and haematological parameters and liver and kidney histologies. Results: Quercetin–3-O-β-glucoside and isorhamnetin–3-O-β-glucoside were the main flavonoids in the HE–Es. Glycemia was reduced by the HE–Es (200 mg/kg b.w., 49.1%) and glibenclamide (10 mg/kg b.w., 52%) treatments. Comparing the antihyperglycemic activities, similar reductions were found between HE–Es (100 mg/kg b.w., 29.32%; 200 mg/kg, 28.99%) and acarbose (10 mg/kg b.w., 19.87%) treatments. On the other hand, the HE–Es was innocuous in mice (LD50 > 1000 mg/kg b.w.), and the results suggested that HE–Es had adaptogenic, and immunostimulant activities. The hepatic and renal histologies were normal; however, male mice showed level zero steatosis that disappeared in the HE–Es satellite after treatment withdrawal. Conclusion: This study reports for the first time the in vivo effects on blood glucose levels of HE–Es and chronic toxicity of a Crassulaceae plant, supporting the antidiabetic potential and safety of HE–Es. Future studies must corroborate the effects of HE–Es in humans, allowing its use in high-value formulations.

10aAntihyperglycemia10aCrassulaceae family10aHypoglycemia10aPlant natural extract10aSafety1 aHeredia-Mercado, Belinda1 aDelgado-Vargas, Francisco1 aOsuna-Martínez, Lorenzo, Ulises1 aNoguera-Corona, Elvic1 aLópez-Valenzuela, José, Ángel1 aRamos-Payán, Rosalio1 aLópez-Angulo, Gabriela uhttps://phcogres.com/article/2023/15/2/105530pres152034