02912nas a2200337 4500008004100000024000700041245013800048210006900186260001800255300001200273490000700285520177600292653002202068653001002090653001102100653000902111653003002120653001202150100003102162700003202193700002902225700002602254700003102280700003102311700003502342700003302377700003902410700003402449700003102483856006002514 2023 eng d a0700aAbrogation of Acute Inflammation in ICR Mice with Topical Administration of Philippine Stingless Bee (Tetragonula biroi Friese) Honey0 aAbrogation of Acute Inflammation in ICR Mice with Topical Admini cFebruary 2023 a258-2660 v153 a

Background: Honey, a sweet sugar-rich bee-derived product, has been widely recognized in the realm of Ayurveda and Pharmacognosy for the treatment of various inflammatory conditions including inflammatory bowel disease, asthma, arthritis, and gastric ulcer. However, the anti-inflammatory effect of Philippine honey obtained from local stingless bees remains unexplored and poorly investigated. Hence, this study delved on the anti-inflammatory potential of topically applied Philippine stingless bee honey (PSH) using the λ-Carrageenan-induced mouse model of paw edema. Materials and Methods: A total of 30, male ICR mice of six weeks of age were randomly divided into the following groups (n=10 per group) as follows: Distilled water-, 1% Diclofenac sodium-, and Honey-treated group. All treatments were administered immediately after Carrageenan injection and then every 8 hr during the 24-hr test duration. Results: Repeated topical application of PSH significantly attenuated the gross hind paw swelling observed at the onset of the 6 hr post-induction (pi) until the 24 hr pi with Carrageenan in contrast to the Distilled water-treated group. This corresponded histologically to a marked reduction in dermal thickness, dermal edema, and leukocytic infiltration. Whereas, at the cytokine level, this correlated to significant suppression of the circulating blood levels of pro-inflammatory cytokines, TNF-α, IL-1β, and IL-6. Conclusion: Based on these results, honey from the Philippine stingless bees shows convincing anti-inflammatory activity which is partly mediated through the regulation of the expression of inflammation-associated cytokines.

10aAnti-inflammatory10aHoney10aIL-1β10aIL-610aPhilippine stingless bees10aTNF-α.1 aDesamero, Mark, Joseph Mar1 aRabino, Mikaela, Bernadette1 aTayao, Maria, Angelica R1 aAng, Mary, Jasmin Cab1 aBariuan, Jussiaea, Valente1 aGapasin, Roxanne, Posilero1 aCollantes, Therese, Marie Aven1 aRondina, Melissa, Marie Riso1 aFernandez-Colorado, Cherry, Pambid1 aCervancia, Cleofas, Rodriguez1 aEstacio, Maria, Amelita Ce uhttps://phcogres.com/article/2023/15/2/105530pres152028