02747nas a2200265 4500008004100000024000700041245013000048210006900178260001700247300001200264490000700276520185000283653002402133653002702157653001902184100003102203700002802234700002702262700002302289700003002312700002402342700002502366700003102391856005902422 2022 eng d a0800aAn HPLC Method to Determine Phenolic Compounds of Plant Extracts: Application to Byrsonima crassifolia and Senna alata Leaves0 aHPLC Method to Determine Phenolic Compounds of Plant Extracts Ap cOctober 2022 a395-4040 v143 a

Background: The Amazonian Region has a variety of medicinal plants with bioactive compounds, whose characterization could present the potential for sustainable development. Objectives: A method for separating, identifying, and quantifying a mixture of nine phenolic compounds (gallic acid, 3-hydroxybenzoic acid, p-coumaric acid, catechin, myricetin, rutin, quercetin, kaempferol, and cyanidin) was developed, validated, and applied to analyze aqueous and hydroethanolic extracts from Byrsonima crassifolia (L.) Kunth and Senna alata (L.) leaves. Materials and Methods: The separation was carried out by HPLC, using a Shim-pack VP-ODS C18 column (5 μm, 150 x 4.6 mm) at 40°C. Detection was performed at 254 nm and separation occurred in 35 min. Results: The optimized method was validated for each of the nine phenolic compounds. The calibration curve for the phenolic compound standards showed suitable linear fitting and exhibited correlation coefficients greater than 0.990. The LOD and LOQ varied between 6.2807 - 14.8851 μg mL-1 and 6.8002 - 16.0071 μg mL-1, respectively. The method was found to be robust for changes of ±2 ml in mobile phase composition. Byrsonima crassifolia aqueous extracts indicated contents of gallic acid, catechin, rutin, and cyanidin whereas hydroethanolic one did not show the first substance. Senna alata aqueous extract presented only 3-hydroxybenzoic acid and rutin whereas myricetin, cyanidin, quercetin, and kaempferol were also identified in the hydroethanolic one. Conclusion: The HPLC method is efficient, precise, accurate, and sensitive to determining phenolic compounds in plant extracts and it is recommended for efficient assays in routine work.

10aBioactive compounds10aChromatographic method10aPlant extracts1 aCavalcante, Marília, de A1 aOliveira, Janyna, dos S1 aBarreto, Mayra, S da S1 aPinheiro, Lucas, P1 aCantuária, Patrick, de C1 aBorges, Wardsson, L1 ada Silva, Gabriel, A1 ade Souza, Tiago, Marcolino uhttps://phcogres.com/article/2022/14/4/105530pres14458