02415nas a2200289 4500008004100000245013600041210007100177260001300248300001200261490000700273520143400280653001301714653001101727653003401738653001001772653002101782653003101803653006001834100002701894700002701921700002601948700002501974700001801999700002602017700002302043856005902066 2020 eng d00aMolecular Identification and Next‑Generation Sequence Analysis of Interspecies Genetic Variations among Three Varieties of Datura0 aMolecular Identification and Next‑Generation Sequence Analysis o cMay 2020 a158-1620 v123 a

Background: Datura is a well‑known plant used in Ayurveda. It is a widely growing plant from Solanaceae family, attributed to poisonous and medicinal values. It is used in the treatment of various skin diseases, fever, etc., It is also used for external application and rarely for oral administration. Objectives: The present study aims to compare three different varieties of Datura which include the species of Datura innoxia Mill., Datura metel L. and Datura arborea are used to study molecular marker and phylogenetic analysis. Materials and Methods: Whole‑genomic DNA was isolated from the leaves of Datura and the polymerase chain reactions amplification of DNA barcoding markers are rbcl, mat k, and internal transcribed spacer‑4 and 5 were analyzed by 0.8% agarose gel electrophoresis. Results: The DNA barcoding markers and next‑generation sequencing are able to identify the interspecies genetic variations among these closely related plant varieties of Datura. Conclusion: The interspecies genetic variations among these closely related three species of D. innoxia, metel, and arborea was closely related with Datura stramonium isolate NN003 chloroplast genome similarity of 98%, 99%, and 99%, respectively.

10aAyurveda10aDatura10aInternal transcribed spacer 410aMat k10aMedicinal plants10aNext-generation sequencing10aRibulose bisphosphate carboxylase/oxygenase form I gene1 aPrasad, Goli, Penchala1 aPratap, Goli, Penchala1 aMarimuthu, Srinivasan1 aPrasad, Shyam, Baboo1 aRao, Gajendra1 aMangal, Anupam, Kumar1 aSrikanth, Naryanam uhttps://phcogres.com/article/2020/12/2/104103prpr10119