02991nas a2200265 4500008004100000245014900041210006900190260001300259300001200272490000700284520199400291653001602285653005602301653001602357653001502373653002302388653013002411100002302541700002602564700001602590700001802606700002502624700001802649856005802667 2020 eng d00aIdentification and Characterization of Polyphenols and Volatile Terpenoid Compounds in Different Extracts of Garden Sage (Salvia officinalis L.)0 aIdentification and Characterization of Polyphenols and Volatile  cMay 2021 a149-1570 v123 a

Introduction: Garden sage (Salvia officinalis L.) is an important medicinal and aromatic herb, used in various food, pharmaceuticals and cosmetic industries for its potential antioxidant properties. Leaves are the source of essential oils and polyphenols, used as a raw material in food and pharmaceutical industries. Materials and Methods: The study aimed to develop a complete phytochemical profile of S. officinalis leaves through liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UHPLC-ESI-QTOF-MS) and gas chromatography with a flame ionization detector (GC-FID). Soxhlet and sonicated extract were analyzed using UHPLC, data-independent acquisition in negative electrospray ionization mode. Essential oil profiling of leaves in comparison with leaves+ stem was carried out using GC-FID. Results: Among the extraction methods, Soxhlet extraction yielded significantly high levels of caffeic acid (92.45 ± 1.92 μg/g), rosmarinic acid (18821.33 ± 150.20 μg/g), luteolin-7-glucoside (635.13 ± 11.20 μg/g), carnosic acid (27.48 ± 2.37 μg/g), carnosol (1347.67 ± 30.04 μg/g), and ursolic acid (14938.67 ± 82.20 μg/g). Among the 43 identified phenolic compounds, two flavonoids, diosmetin and pectolinarigenin and two triterpenoids benthamic and micromeric acids have been first time detected in S. officinalis leaves. The results of essential oil analysis indicated the presence of α-thujone (34.43- 38.93 %), β-thujone (6.03-7.58 %), camphor (15.77-18.12 %), 1,8-cineole (5.45-6.21 %), α-humulene (5.20 %), and camphene (4.29-5.10 %) as major volatile terpenoid components in S. officinalis. Conclusion: Soxhlet extraction found to be the best method for polyphenol extraction and the essential oil extracted only from leaves best suitable for therapeutic purposes due to less α-thujone and β-thujone content.

10aGarden sage10aGas chromatography with a flame ionization detector10aPolyphenols10aSonication10aSoxhlet extraction10aUltra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry1 aSharma, Yashaswini1 aVelamuri, Ravikishore1 aFagan, John1 aSchaefer, Jim1 aStreicher, Christoph1 aStimson, John uhttps://phcogres.com/article/2020/12/2/104103prpr9219