@article {291, title = {The Hydroalcoholic Extract of Matricaria chamomilla Suppresses Migration and Invasion of Human Breast Cancer MDA-MB-468 and MCF-7 Cell Lines}, journal = {Pharmacognosy Research}, volume = {9}, year = {2021}, month = {February 2017}, pages = {87-95}, type = {Original Article}, chapter = {87}, abstract = {

Background: Matricaria chamomilla is an aromatic plant with antioxidant, anticancer, and anti-inflammatory properties. However, the inhibitory role of M. chamomilla on migration and invasion of human breast cancer cells remains unclear. gThis study investigated the methods to evaluate these anticancer mechanisms of M. chamomilla on human breast cancer MCF-7 and MDA-MB-468 cell lines. Materials and Methods: The cells were treated with hydroalcoholic extract of M. chamomilla at different concentrations (50{\textendash}1300 μg/mL) for 24, 48, and 72 h in a culture medium containing 10\% fetal bovine serum. This study quantified the 50\% growth inhibition concentrations (IC50) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay; apoptosis and necrosis through Hoechst 33342/propidium iodide staining; cell proliferation and clone formation by clonogenic assay as well as cellular migration, invasion, and attachment. After 24, 48, and 72 h of treatment, the IC50 levels were 992 {\textpm} 2.3 μg/mL, 893 {\textpm} 5.4 μg/ mL, and 785 {\textpm} 4.8 μg/mL against MDA-MB-468, respectively, and 1288 {\textpm} 5.6 μg/mL, 926 {\textpm} 2.5 μg/mL, and 921 {\textpm} 3.5 μg/mL, against MCF-7, respectively. Furthermore, increasing the extract concentrations induced cellular apoptosis and necrosis and decreased cellular invasion or migration through 8 μm pores, colonization and attachment in a dose-dependent manner. Results: It indicated time- and dose-dependent anti-invasive and antimigrative or proliferative and antitoxic effects of hydroalcoholic extract of aerial parts of chamomile on breast cancer cells. Conclusion: This study demonstrated an effective plant in preventing or treating breast cancer.

}, keywords = {Apoptosis, Attachment, Breast cancer, Invasive, Migration}, doi = {10.4103/0974-8490.199778}, author = {Mohsen Nikseresht, and Ali Mohammad Kamali and Hamid Reza Rahimi and Hamdollah Delaviz and Mehdi Akbartabar Toori and Iraj Ragerdi Kashani and Reza Mahmoudi} } @article {752, title = {Screening of Anticancer Activity from Agarwood Essential Oil}, journal = {Pharmacognosy Research}, volume = {6}, year = {2014}, month = {May,2014}, pages = {191-194}, type = {Original Article}, chapter = {191-194}, abstract = {

Background: Agarwood is a priceless non-timber forest product from Aquilaria species belonging to the Thymelaeaceae family. As a result of a defence mechanism to fend off pathogens, Aquilaria species develop agarwood or resin which can be used for incense, perfumery, and traditional medicines. Evidences from ethnopharmacological practices showed that Aquilaria spp. have been traditionally used in the Ayurvedic practice and Chinese medicine to treat various diseases particularly the inflammatory-associated diseases. There have been no reports on traditional use of agarwood towards cancer treatment. However, this is most probably due to the fact that cancer nomenclature is used in modern medicine to describe the diseases associated with unregulated cell growth in which inflammation and body pain are involved. Objective: The aim of this current study was therefore to investigate the potential anticancer properties of agarwood essential oil obtained from distillation of agarwood (resin) towards MCF-7 breast cancer cells. Materials and Methods: The essential oil was subjected to screening assays namely cell viability, cell attachment and sulforhodamine B (SRB)-based cytotoxicity assay to determine the IC50 value. Results: The agarwood essential oil caused reduction of the cell number in both the cell viability and attachment assay suggesting a cumulative effect of the cell killing, inhibition of the cell attachment and or causing cells to detach. The agarwood essential oil showed IC50 value of 900 μg/ml towards the cancer cells. Conclusion: The agarwood essential oil exhibited anticancer activity which supports the traditional use against the inflammatory-associated diseases. This warrants further investigation towards the development of alternative remedy towards cancer.

}, keywords = {Agarwood, Aquilaria, Breast cancer, MCF-7, Resin, SRB}, doi = {10.4103/0974-8490.132593}, author = {Yumi Zuhanis Has-Yun Hashim and Abbas Phirdaous and Amid Azura} }