@article {251, title = {Antihyperglycemic Activity of the Leaves from Annona cherimola Miller and Rutin on Alloxan-induced Diabetic Rats}, journal = {Pharmacognosy Research}, volume = {9}, year = {2017}, month = {February 2017}, pages = {1-6}, type = {Original Article}, chapter = {1}, abstract = {

Background: Annona cherimola, known as {\textquotedblleft}chirimoya{\textquotedblright} has been reported in Mexican traditional medicine for the treatment of diabetes. Objective: The aims of the present study were to validate and assess the traditional use of A. cherimola as an antidiabetic agent. Materials and Methods: The ethanol extract from A. cherimola (300 mg/ kg, EEAc), subsequent fractions (100 mg/kg), and rutin (30 mg/kg) were studied on alloxan-induced type 2 diabetic (AITD) and normoglycemic rats. In addition, oral glucose tolerance test (OGTT) and oral sucrose tolerance test (OSTT) were performed in normoglycemic rats. Molecular docking technique was used to conduct the computational study. Results: Bioassay-guided fractionation of EEAc afforded as major antihyperglycemic compound, rutin. EEAc attenuated postprandial hyperglycemia in acute test using AITD rats (331.5 mg/dL) carrying the glycemic levels to 149.2 mg/ dL. Rutin after 2 h, attenuated postprandial hyperglycemia in an acute assay using AITD rats such as EEAc, with maximum effect (150.0 mg/dL) being seen at 4 h. The antihyperglycemic activities of EEAc and rutin were comparable with acarbose (151.3 mg/dL). In the subchronic assay on AITD rats, the EEAc and rutin showed a reduction of the blood glucose levels since the 1st week of treatment, reaching levels similar to normoglycemic state (116.9 mg/kg) that stayed constant for the rest of the assay. OGTT and OSTT showed that EEAc and rutin significantly lowered blood glucose levels in normoglycemic rats at 2 h after a glucose or sucrose load such as acarbose. Computational molecular docking showed that rutin interacted with four amino acids residues in the enzyme α-glucosidase. Conclusion: The results suggest that rutin an α-glucosidase inhibitor was responsible in part of the antihyperglycemic activity of A. cherimola. Its in vivo antihyperglycemic activity is in good agreement with the traditional use of A. cherimola for the treatment of diabetes.

}, keywords = {Annona cherimola Miller, Annonaceae, Rutin, Type 2 diabetes mellitus, α-Glucosidase}, doi = {10.4103/0974-8490.199781}, author = {Fernando Calzadax and Fernando Calzada and Jes{\'u}s Iv{\'a}n Solares-Pascasio and RM Ordo{\~n}ez-Razox and RM Ordo{\~n}ez-Razo and Claudia Velazquez and Elizabeth Barbosa and Normand Garc{\'\i}a-Hern{\'a}ndez and David Mendez-Luna and Jos{\'e} Correa-Basurto} } @article {773, title = {Cytotoxic Acetogenins from Annona glabra Cultivated in Egypt}, journal = {Pharmacognosy Research}, volume = {1}, year = {2009}, month = {January 2010 }, pages = {130-135}, type = {Original Article}, chapter = {130}, abstract = {

Bio-assay guided fraction of the methanolic extract of Annona glabra seeds (Annonaceae), cultivated in Egypt, revealed to the isolation of three bis-tetrahydrofuran acetogenins; squamocin-C (1), squamocin-D (2), and annonin I (3). Compounds 1 and 2 were obtained as stereoisomeric mixture. All isolates were assayed for their cytotoxicity twards brine shrimp and five in vitro cancer cell lines (A549, HT29, MCF 7, RPMI, and U251), and showed significant activity The structures of all compounds were determined by interpretation of their NMR and MS analyses.

}, keywords = {Acetogenin, Annona glabra, Annonaceae, Brine shrimp, Cytotoxic}, doi = {Nil}, author = {Ahmed Abdel-Lateff and Bassem S. El-Menshawi and Mohamed Y. Haggag and Mahmoud A. Nawwar} }