@article {230, title = {Method Development and Validation for the Determination of Caffeine: An Alkaloid from Coffea arabica by High-performance Liquid Chromatography Method}, journal = {Pharmacognosy Research}, volume = {10}, year = {2018}, month = {February 2018}, pages = {88-91}, type = {Original Article}, chapter = {88}, abstract = {

Objective: The present study was investigated to develop and validate a reversed phase high performance liquid chromatography method for the determination of caffeine from bean material of Coffee arabica. Materials and Methods: The separation was achieved on a reversedphase C18 column using a mobile phase composed of water: methanol (50:50) at a flow rate of 1.0 mlmin-1. The detection was carried out on a UV detector at 272 nm. The developed method was validated according to the requirements for International Conference on Harmonisation (ICH) guidelines, which includes specificity, linearity, precision, accuracy, limit of detection and limit of quantitation. Results: The developed method validates good linearity with excellent correlation coefficient (R2 \> 0.999). In repeatability and intermediate precision, the percentage relative standard deviation (\% RSD) of peak area was less than 1\% shows high precision of the method. The recovery rate for caffeine was within 98.78\% - 101.28\% indicates high accuracy of the method. The low limit of detection and limit of quantitation of caffeine enable the detection and quantitation of caffeine from C. arabica at low concentrations. Conclusion: The developed HPLC method is a simple, rapid, precisely, accurately and widely accepted and it is recommended for efficient assays in routine work.

}, keywords = {Caffeine, Coffea arabica, Linearity, Precision, Recovery}, doi = {10.4103/pr.pr_79_17}, author = {P Naveen and HB Lingaraju and M Deepak and B Medhini and K Shyam Prasad} } @article {431, title = {Validation of High-Performance Liquid Chromatography Method for Quantification of Costunolide Content in Laurus nobilis}, journal = {Pharmacognosy Research}, volume = {10}, year = {2018}, month = {October 2018}, pages = {422-425}, type = {Original Article}, chapter = {422}, abstract = {

Objective: To establish a method to determine the content of costunolide in Laurus nobilis leaves by high-performance liquid chromatography (HPLC). Materials and Methods: The separation was performed on a reversed-phase C18 column (100 {\r A}, 150 mm {\texttimes} 4.6 mm, 5 μm pore size) using a mobile phase composed of water:acetonitrile (40:60) at a flow rate of 1.0 ml/min. The detection was carried out on a ultraviolet detector at 210 nm. The developed method was validated according to the requirements for International Conference on Harmonisation guidelines. Results: The proposed method for costunolide was validated for linearity with excellent correlation coefficient (r2 \> 0.999). The relative standard deviation (RSD) is less than 1\% in precision (i.e repeatability and intermediate) of the method. The recovery rate for costunolide was within 100.54\%{\textendash}102.62\%. The limit of detection and limit of quantitation were 2.29 and 6.64 parts per million, respectively. Conclusion: The developed HPLC method is simple, rapid, precisely, accurately, and widely accepted and it is recommended for efficient assays in routine work.

}, keywords = {Costunolide, Laurus nobilis, Linearity, Precision, Recovery, Validation}, doi = {10.4103/pr.pr_31_18}, author = {Puttaswamy Naveen and Harakanahalli Basavegowda Lingaraju1 and Siddappa Chandrappa1 and Koretekera Shekharappa Priya, and Kodimule Shyam Prasad} }