@article {1300, title = {Exploring the Antioxidant and Antiproliferative Properties of Four Medicinal Plants from Veracruz, Mexico for Urinary and Prostate Health}, journal = {Pharmacognosy Research}, volume = {16}, year = {2024}, month = {October 2024}, pages = {817-827}, type = {Original Article}, chapter = {817}, abstract = {

Background: C. scaber, P. aequale, S. oleraceus and S. racemosa play a vital role in traditional medicine in Veracruz, Mexico, known for their effectiveness in treating a wide range of health issues. Particularly noteworthy is their significant use in traditional medicine in Mexico for addressing urinary and prostate-related ailments. Objectives: The current study assesses the antioxidant and anti-proliferative properties of these species on the androgen-independent prostate cancer cell line PC-3. Materials and Methods: Methanolic and aqueous extracts of the selected plants{\textquoteright} parts were prepared and their chemical profiles were evaluated using 1H-RMN. Antioxidant activity was assessed using the DPPH and FRAP methods. The total phenolic content was determined using the Folin-Ciocalteu method. The antiproliferative activity on PC-3 and HPrEC cells was evaluated by the MTT method. Results: S. racemosa showed the highest antioxidant activity (84.90\% radical scavenging activity), attributed to the presence of flavonoids. However, the methanol extract of P. aequale had a better effect on the proliferation of PC-3 cells with an IC50 of 251.6 {\textmu}g/mL, additionally, it showed cytotoxicity at high concentrations (above 500 {\textmu}g/mL). The antiproliferative and cytotoxic effect observed is not associated with antioxidant activity or the presence of alkaloids. However, cytotoxicity also occurred in control cells (HPrEC) with an IC50 of 23.98 {\textmu}g/mL. Conclusion: The methanolic extract of P. aequale showed the highest antiproliferative activity against PC-3 cell line, an effect not related to its antioxidant activity and attributed to the presence of aliphatic, aromatics and aldehydes compounds identified by 1H-NMR in the extracts.

}, keywords = {Antioxidant activity, Antiproliferative activity, Costus scaber, Piper aequale, Serjania racemosa, Sonchus oleraceus}, doi = {10.5530/pres.16.4.94}, author = {Oscar Antonio S{\'a}nchez-Aguirre and Cynthia Fern{\'a}ndez-Pomares and Enrique Ju{\'a}rez-Aguilar and Alberto S{\'a}nchez-Medina and Leticia Margarita Cano-Asseleih} } @article {225, title = {Antiproliferative and Apoptotic Activities of the Medicinal Plant Ziziphus obtusifolia}, journal = {Pharmacognosy Research}, volume = {10}, year = {2018}, month = {February 2018}, pages = {55-59}, type = {Original Article}, chapter = {55}, abstract = {

Background: Ziziphus obtusifolia is a spiny shrub found in Northwest Mexico desert, with traditional medicinal use to treat several diseases including cancer. Objective: The aims of the present study were to evaluate the antiproliferative and apoptotic activities of the aerial parts of this plant. Materials and Methods: The methanol extract and its fractions were prepared using several solvents. The antiproliferative activity was evaluated by the (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium) (MTT) assay on HeLa, A549, RAW 264.7, M12.C3.F6, and L-929 cell lines, and the apoptotic activity using Annexin V and (5,50,6,60-tetra-chloro-1,10,3,30-tetra-ethylbenzimidazol-carbocyanine iodide) staining. The most active fraction was further separated by column chromatography. Results: The most active fraction was hexane with an IC50 of 90.4 {\textmu}g/mL against RAW 264.7, 94 {\textmu}g/mL against M12. C3.F6, 165.5 {\textmu}g/mL against HeLa and 187.7 {\textmu}g/mL against A549 cell lines. In apoptotic activity assays the methanol extract and its n-hexane fraction were found to induce mitochondrial depolarization in HeLa cells (83 and 87\% respectively), and both induced the externalization of the phosphatidylserine increasing the percentage of cells in early apoptosis from 1.4\% in untreated control cells, to 1.9\% and 3.5\% for methanol extract and n-hexane fraction-treated cells, respectively, statistically different for the total percentage of apoptotic cells (P \< 0.05). Conclusions: These results show that Z. obtusifolia has antiproliferative a

}, keywords = {Antiproliferative activity, Apoptotic activity, Ziziphus obtusifolia}, doi = {10.4103/pr.pr_38_17}, author = {Edna D. Molina-Romo and Adriana Garibay-Escobar and Olivia Valenzuela-Antelo and Eduardo Ruiz-Bustos and Javier Hern{\'a}ndez Mart{\'\i}nez and Carlos V{\'e}lazquez and Luisa A. Rasc{\'o}n-Valenzuela and Ram{\'o}n Enrique Robles-Zepeda} } @article {717, title = {In vitro antiproliferative activity of Annona reticulata roots on human cancer cell lines}, journal = {Pharmacognosy Research}, volume = {3}, year = {2011}, month = {April 2011}, pages = {09-12}, type = {Original Article}, chapter = {09}, abstract = {

Background:\ The phytochemical and pharmacological activities of\ Annona reticulata\ components suggest a wide range of clinical application in lieu of cancer chemotherapy.\ Materials and Methods:\ Ethanol and aqueous extracts of roots of\ Annona reticulata\ Linn were studied for their\ in vitro\ antiproliferative activity on\ A-549\ (human lung carcinoma),\ K-562\ (human chronic myelogenous leukemia bone marrow), HeLa\ (human cervix) and\ MDA-MB\ (human adenocarcinoma mammary gland) cancer cell lines by MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay.\ Results:\ The ethanol extract exhibited a prominent inhibitory effect against\ A-549, K-562, HeLa\ and\ MDA-MB\ human cancer cell lines at a concentration range between 10 and 40 μg/ml, whereas the aqueous extract showed a lower activity at the same concentration. Simultaneously, the effect of the ethanol extract toward the inhibition of\ Vero\ cell line proliferation was lower in comparison with the cancer cell lines.\ Conclusion:\ The significant antiproliferative activity of the ethanol extract of\ Annona reticulata\ roots against\ A-549, K-562, HeLa\ and\ MDA-MB\ human cancer cell lines may be attributed toward the collective presence of acetogenins, alkaloids and lower inhibitory effect on\ Vero\ cell line, which suggests\ Annona reticulata\ be used as a chemopreventive agent in cancer therapy.

}, keywords = {A-549, Annona reticulata, Antiproliferative activity, HeLaand MDA-MBhuman cancer cell lines, K-562, MTT assay}, doi = {10.4103/0974-8490.79109}, author = {HM Suresh and B Shivakumar and K Hemalatha and SS Heroor and DS Hugar and K.R.S Sambasiva Rao} }