@article {218, title = {Quantitative Phytochemical Constituents and Antioxidant Activities of the Mistletoe, Phragmanthera capitata (Sprengel) Balle Extracted with Different Solvents}, journal = {Pharmacognosy Research}, volume = {10}, year = {2018}, month = {February 2018}, pages = {16-23}, type = {Original Article}, chapter = {16}, abstract = {

Objective: The investigation aimed to evaluate the in vitro quantitative phytochemical compositions and antioxidant capacity of the dried leaves of Phragmanthera capitata extracted with different solvents. Methodology: Different phytochemical assays were used to evaluate the phenols, flavonoids, and proanthocyanidin contents of the acetone, methanol, ethanol, and aqueous extracts of the sample. Antioxidant activity was measured using 2,2diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), Ferric reducing antioxidant power (FRAP), nitric oxide, and total antioxidant capacity (TAC) assays. Results: Methanol had the highest extraction yield (21.50\%) followed by aqueous (10.87\%). Acetone and ethanol yielded 6.87\% and 4.3\%, respectively. However, acetone and ethanol extracts had more phenolic content (218.6 {\textpm} 3.38 and 214.8 {\textpm} 3.56 mg gallic acid equivalent (GAE)/g, respectively) that were significantly higher (P \< 0.05) than the methanol and aqueous extracts (203.8 {\textpm} 1.95 and 175.5 {\textpm} 0.32 mg GAE/mg, respectively). Acetone extract also had more flavonoid and proanthocyanidin contents (679.8 {\textpm} 6.26 mg quercetin equivalent/g and 645.7 {\textpm} 6.35 mg catechin equivalent/g, respectively) that were significantly higher than the other solvent extracts (P \< 0.05). All the solvent fractions showed great antioxidant activities with the acetone fraction having the highest capacity based on ABTS, DPPH, and TAC assays (half maximal inhibitory concentration [IC50] = \<5 {\textmu}g/ mL, 24.5 {\textmu}g/mL, and 85 {\textmu}g/mL respectively). The methanol extract however had FRAP and nitric oxide antioxidant activities (IC50 = 302 {\textmu}g/ mL and \<25 {\textmu}g/mL respectively). Conclusion: The findings of this study provide evidence that P. capitata has potential as a natural source of antioxidant with acetone showing to be the best extractant of the polyphenolics with corresponding higher antioxidant activities.

}, keywords = {Extractant, Loranthaceae, Mistletoe, Phragmanthera capitata, Polyphenolics, Total antioxidant activity}, doi = {10.4103/pr.pr_65_17}, author = {Franklin Uangbaoje Ohikhena and Olubunmi Abosede Wintola and Anthony Jide Afolayan} }