@article {198, title = {The Cellular Activities of the Subfraction of Red Onion Peel Crude Ethanolic Extract in MDA-MB-231 Cells}, journal = {Pharmacognosy Research}, volume = {12}, year = {2020}, month = {January 2021}, pages = {416-423}, type = {Original Article}, chapter = {416}, abstract = {

Aims: This study aimed to evaluate the cellular activities of a subfraction (F1) of red onion peel crude ethanolic extract in MDA-MB-231. Materials and Methods: The cell cycle profile and apoptosis induction in F1-treated MDA-MB-231 were measured using flow cytometry, and the migratory activity was measured using wound healing assay, Boyden chamber motility assay, and Matrigel chamber invasion assay, whereas other related protein expressions were measured using Western blotting. Results: Our results demonstrated that the treatment of MDA-MB-231 with F1 reduced cell proliferation of the cells via inducing cell cycle arrest at S and G2 phases. F1 also possessed a better cell cycle arrest profile than camptothecin and executed better cell migratory effect in MDA-MB-231, which was likely via inhibition of the Akt signaling pathway in MDA-MB-231. Conclusion: F1 may have the potential to be developed as a nutraceutical agent that promotes anticancer effects or to be used in combination with other chemotherapy drugs to reduce the toxicity of drugs for MDA-MB-231-type breast cancer treatment.

}, keywords = {Akt signaling pathway, Cell cycle, Cell migratory activity, Cellular activity, MDA-MB-231, Red onion peel}, doi = {10.4103/pr.pr_20_20}, author = {Sin Pei Choe and Poh Chiew Siah and Shern Kwok Lim and Kok Khiang Peh and Ming Thong Ong and Boon Yin Khoo} } @article {801, title = {Antiproliferative Activity and Induction of Apoptosis in Estrogen Receptor-positive and Negative Human Breast Carcinoma Cell lines by Gmelina asiatica Roots}, journal = {Pharmacognosy Research}, volume = {2}, year = {2010}, month = {May 2010}, pages = {113-119}, type = {Original Article}, chapter = {113}, abstract = {

Low risk of breast cancer has been proposed to be associated with high intake of lignans. We have reported the presence of lignans in Gmelina asiatica roots. There are no scientific reports on the antiproliferative activity of G. asiatica roots. The objective of the present study was to evaluate the effect of ethyl acetate extract from G. asiatica roots (EGAR) on estrogen receptor-positive (MCF-7) and negative (MDA-MB-231) human breast cancer cell lines. The effects of 50\% inhibitory concentrations (IC50) of EGAR on MCF-7 and MDA-MB-231 cells were determined using 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay kit. The mode of cell death caused by EGAR was determined using dual apoptosis assay kit by observing the cells under fluorescent microscope. The quantification of apoptosis and necrosis in cells caused by EGAR was determined using cell death detection kit through ELISA. Down-regulation of the proliferative activity occurred in a clear dose-dependent response with IC50 values of 32.9 {\textpm} 3.8 μg/mL in MCF-7 and 19.9 {\textpm} 2.3 μg/ mL in MDA-MB-231 cell lines. Treatment of breast cancer cells with EGAR resulted in significant apoptosis. The EGAR contain lignans and flavonoids. The antiproliferative activity of the extract is attributed to the presence of these secondary metabolites. The results suggest the efficacy of G. asiatica roots as antiproliferative agents on human breast cancer cells, supporting the hypothesis that plants containing lignans have beneficial effects on human breast cancer.

}, keywords = {Annexin-V, Caspase-3, Enrichment factor, Lignans, MCF-7, MDA-MB-231, Phosphatidylserine}, doi = {10.4103/0974-8490.62949}, author = {Madhu Katyayani Balijepalli and Satyanarayana Tandra and Mallikarjuna Rao Pichika} }