@article {1397, title = {Bioactive Compounds in Inocutis levis and their Cytotoxic Effects on MCF-7 and HT-29 Cancer Cells}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {April 2025}, pages = {608-618}, type = {Original Article}, chapter = {608}, abstract = {

Background and Objectives: Fungi are undeniable sources of all types of secondary metabolites and bioactive compounds. Inocutis levis is a polypore fungus belonging to Hymenochaetales of basidiomycetes. This study aimed to identify the compounds in the extract and investigate the cytotoxicity effect of Inocutis levis on different cell lines (MCF-7, HT-29 and HFF). Materials and Methods: The extract was obtained by rotary evaporator and LC/MS was used to identify the compounds in the extract. The cytotoxic effect of Inocutis levis against MCF-7, HT-29 and HFF cells was evaluated using MTT assay, 24 and 48 hr after the addition of the extract. Also, flow cytometry analysis was carried out to study the cell cycle arrest and apoptosis.Results: The LC/ MS assay data showed that the mushroom extract contains antioxidant compounds. Based on the MTT test, Inocutis levis extract did not decrease the viability of non-cancerous HFF cells. Still, it was able to inhibit the growth and increase the death in HT-29 cells at a concentration of 100 μg/mL after 24 and 48 hr (p\<0.05) also, MCF-7 cells at concentrations of 1 and 10 and 100 μg/ mL after 24 and 48 hr (p\<0.05). Evaluation of the cell cycle 48 hr after adding the extract by flow cytometry, showed that the mushroom extract induced apoptosis in MCF-7 cells. Conclusion: Inocutis levis contains antioxidant compounds and can inhibit the growth of HT-29 and especially MCF-7 cancer cells via the induction of apoptosis.

}, keywords = {anticancer, Apoptosis, Cell cycle, HT-29, Inocutis levis, LC/MS, MCF-7}, doi = {10.5530/pres.20252069}, author = {Fereshteh Mir Mohammad Rezaei and Fatemeh Tavakoli and Maryam Kiaei and Vahid Ramezani and Amin Zarezadeh and Masoumeh Ghobad-Nejhad} } @article {1215, title = {Evaluation of the Effect of Five Colombian Propolis Extracts on the Expression of Genes Associated with Cell Cycle and Inflammation in a Canine Osteosarcoma Cell Line}, journal = {Pharmacognosy Research}, volume = {16}, year = {2023}, month = {December 2023}, pages = {172-182}, type = {Original Article}, chapter = {172}, abstract = {

Introduction: Propolis has anti-inflammatory, antitumor, antibacterial and immunomodulatory properties, which is why it is suggested that it could be used as an alternative or complementary drug therapy in the treatment of various pathologies such as cancer, diseases chronic-degenerative and infectious. Materials and Methods: In this study, canine bone fibroblasts were used as control cells, and the canine Osteosarcoma cell line (OSCA-8) was acquired to evaluate the effects of ethanol extracts of propolis from five Colombian regions on these cells. The cytotoxic effect was evaluated by examining changes in cell viability and proliferation. Furthermore, the expression of some relevant and characteristic genes related to the tumor phenotype, related to the proinflammatory process and cell cycle, was assessed. Results: Thus, the evaluation of the relative expression of some genes associated with the cell cycle and inflammation could improve the understanding of the cytotoxic effect of propolis extracts on OSCA-8 cell lines. Conclusion: The first time in Colombia, the biological activity of ethanol extracts of propolis was evaluated regarding the inflammation and cell cycle pathways. After 48 hr, the Colombian EEP had an effect on the increase in both OSCA-8 cells and fibroblasts.

}, keywords = {Biological activity, Cell cycle, Gene expression, Inflammation, Osteosarcoma, Propolis.}, doi = {10.5530/pres.16.1.22}, author = {Oscar Juli{\'a}n Murillo Torres and Dolly Patricia Pardo Mora and Orlando Torres Garc{\'\i}a and Mauricio Rey Buitrago} } @article {64, title = {Anticancer Activity of Ethyl Acetate Extract from Lethariella cladonioides in vitro and in vivo}, journal = {Pharmacognosy Research}, volume = {12}, year = {2020}, month = {February 2020}, pages = {40-46}, type = {Original Article}, chapter = {40}, abstract = {

Background: Lethariella cladonioides, also known as Red Snow Tea and Golden Silk Tea, mainly grows in Tibet, Sichuan and Yunnan of China. Its medicinal value has been gradually discovered. Objectives: This study mainly evaluated the anti-cancer effect of AMH-L extracted from L. cladonioides in vitro and in vivo and to explore its anticancer mechanism. Materials and Methods: MTT assay was used to determine the cytotoxicity of AMH-L to human cancer cells A-549 and HCT-116 in vitro. Sarcoma S-180 and human lung cancer NCL-H460 cells were implanted into Kunming mice and Balb/C nude mice, respectively, anti-cancer activity in vivo was assessed by recording body weight, organ coefficient and tumor inhibition rate. Flow cytometry and western blotting were used to preliminarily explore the anticancer mechanism. Results: AMH-L, the active component of Red Snow Tea, had strong anticancer activity against A-549 and HCT-116, and IC50 was 26.17 {\textpm} 3.54 and 16.21 {\textpm} 0.83. When the dosage was 50, 100 and 150 mg/ kg, the inhibition rates of S-180 in Kunming mice were 36.04\%, 43.14\%, and 50.45\% in turn, respectively. At the end of the experiment, the dose was 150, and the relative proliferation inhibition rate of Balb/C nude mice NCI-H460 tumor was 50.36\%. Cell cycle and apoptotic results showed that AMH-L could block A-549 at G2/M phase and promote apoptosis of HCT-116 by inducing poly (ADP-ribose) polymerase lysis. Conclusion: L. cladonioides extract AMH-L exhibits strong anticancer activity in vivo and in vitro and can be used as a potential anticancer drug for further study.

}, keywords = {Anticancer drug, Apoptosis, Cell cycle, Cleaved poly (ADP-ribose) polymerase, Lichen}, doi = {10.4103/pr.pr_70_19}, author = {Wen-Jing Lian and Xiao-Qiong He and Qian Yao and Zhong-Yu Song and Jing Wang} } @article {198, title = {The Cellular Activities of the Subfraction of Red Onion Peel Crude Ethanolic Extract in MDA-MB-231 Cells}, journal = {Pharmacognosy Research}, volume = {12}, year = {2020}, month = {January 2021}, pages = {416-423}, type = {Original Article}, chapter = {416}, abstract = {

Aims: This study aimed to evaluate the cellular activities of a subfraction (F1) of red onion peel crude ethanolic extract in MDA-MB-231. Materials and Methods: The cell cycle profile and apoptosis induction in F1-treated MDA-MB-231 were measured using flow cytometry, and the migratory activity was measured using wound healing assay, Boyden chamber motility assay, and Matrigel chamber invasion assay, whereas other related protein expressions were measured using Western blotting. Results: Our results demonstrated that the treatment of MDA-MB-231 with F1 reduced cell proliferation of the cells via inducing cell cycle arrest at S and G2 phases. F1 also possessed a better cell cycle arrest profile than camptothecin and executed better cell migratory effect in MDA-MB-231, which was likely via inhibition of the Akt signaling pathway in MDA-MB-231. Conclusion: F1 may have the potential to be developed as a nutraceutical agent that promotes anticancer effects or to be used in combination with other chemotherapy drugs to reduce the toxicity of drugs for MDA-MB-231-type breast cancer treatment.

}, keywords = {Akt signaling pathway, Cell cycle, Cell migratory activity, Cellular activity, MDA-MB-231, Red onion peel}, doi = {10.4103/pr.pr_20_20}, author = {Sin Pei Choe and Poh Chiew Siah and Shern Kwok Lim and Kok Khiang Peh and Ming Thong Ong and Boon Yin Khoo} }