@article {1315, title = {Melezitose a Potential of Bioactive Compound from Aqueous Extract of Indian Satavari: An Web Based in silico Study Utilizing GC-MS Analysis}, journal = {Pharmacognosy Research}, volume = {16}, year = {2024}, month = {October 2024}, pages = {887-896}, type = {Original Article}, chapter = {887}, abstract = {

Background: Historical records indicate that medicinal plants and their components have been utilized for treating diverse conditions since antiquity. Asparagus racemosus, in particular, demonstrates a broad spectrum of therapeutic possibilities. Aim: This study aimed to pinpoint potential bioactive compounds found within the aqueous extract of Asparagus racemosus. Materials and Methods: Gas Chromatography employed to identify the presence of various molecules, while Mass Spectrometry and FTIR analysis validated their molecular structures. The molecule was subjected to thorough scrutiny for its potential as a drug candidate, its biological activity, and its predicted targets using in silico techniques. Results: The aqueous extract stands out as the richest reservoir of carbohydrates and steroidal alkaloids. The molecule Melezitose was found in the highest concentration (37.86\%). The identified molecule is a carbohydrate in nature. The computational analysis revealed promising drug-like properties and therapeutic potential for the investigated molecule. Conclusion: It could serve as a viable therapeutic option for addressing multiple disorders.

}, keywords = {Aqueous extract, Asparagus racemosus, Chromatography, In silico study, Mass spectroscopy, Target analysis}, doi = {10.5530/pres.16.4.101}, author = {Sandip Sen and Kalepu Swathi and Dubbasi Vishwanath and Bairam Ravindar and Mitta Chaitanya and Srinivas Cherukupally and Shapuram Tejasree and Hema Varaha Santhoshi Allu} } @article {1214, title = {In silico Based Virtual Screening of Non-polar Phytochemicals obtained From Petroleum Ether Extract of Asparagus racemosus by GC-MS Analysis}, journal = {Pharmacognosy Research}, volume = {16}, year = {2023}, month = {December 2023}, pages = {161-171}, type = {Original Article}, chapter = {161}, abstract = {

Background: Evidence shows that people have used medicinal plants and plant components to treat various conditions since ancient times. Asparagus racemosus have a wide range of therapeutic potential Antioxidant, anti-cancer, anti-fungal, anti-bacterial, and anti-inflammatory. Aim and Objectives: The current study sought to identify potential bioactive molecules present in the petroleum ether extract of Asparagus racemosus. Materials and Methods: The presence of different molecules was identified by Gas Chromatography. Mass spectroscopy and FTIR analysis confirmed the molecular structure. The identified molecule was analyzed for drug likeliness, bioactivity, and target prediction analysis by in silico approaches using Swiss ADME, Swiss target prediction, Oasis datawarior, and mole inspiration software. Results: The petroleum ether extract is the highest source of alkaloids and steroids. The molecule (5.beta.) Pregnane-3,20, beta.-diol, 14.alpha.,1, 8. alpha.-[4-methyl-3-oxo-(1-oxa-4-azabutane-1,4-diyl)]- diacetate was found in the highest concentration (37.33\%). The identified molecule is steroidal. The in silico study exhibited good drug likeliness and therapeutic potential for the reported molecule. The molecule can be considered a potential therapeutic agent for the treatment of cancer and analgesic anti-inflammatory agents. Conclusion: The molecule (5.beta.) Pregnane-3, 20. beta.-diol, 14.alpha.,1, 8. alpha.- [4-methyl-3-oxo-(1-oxa-4-azabutane-1,4-diyl)]-,diacetate can be considered as poteintial therapeutic agent.

}, keywords = {Asparagus racemosus, Drug likeliness, Gas chromatography, In silico study, Mass spectroscopy, Petroleum ether, Target prediction.}, doi = {10.5530/pres.16.1.21}, author = {Shaik Harun Rasheed and Ravi Kumar Vakkalagadda and Sayyad Mustak and Sandip Sen} } @article {186, title = {Larvicidal Efficacy of Andrographis paniculata and Tinospora cordifolia against Aedes aegypti: A Dengue Vector}, journal = {Pharmacognosy Research}, volume = {12}, year = {2020}, month = {January 2021}, pages = {352-360}, type = {Original Article}, chapter = {352}, abstract = {

Background: Aedes aegypti, dengue fever mosquito, is predominantly accompanying the transmission of dengue in tropical and subtropical countries of the world, causing millions of deaths every year. Materials and Methods: The current research was carried out to evaluate the larvicidal efficiency of Andrographis paniculata and Tinospora cordifolia against third instar larvae of A. aegypti using in silico and experimental study. Results: The methanolic extract of A. paniculata and ethanolic extract of T. cordifolia showed the highest larvicidal activity with LC50 and LC90 value of 15.75 ppm; 349.60 and 100.64 ppm; 386.37 ppm, respectively after 48 h exposure, which was statistically significant (P = 0.05). However, the combined action of methanolic extract of A. paniculata + ethanolic (50:50) extract of T. cordifolia and the bioactive fraction of methanolic extract of A. paniculata showed the effective larvicidal activity with LC50 value of 113.20 ppm and 236.08 ppm respectively after 24 h treatment which was statistically significant (P = 0.05). The m/z values in liquid chromatography-mass spectrometry graph of bioactive fraction of methanolic extract of A. paniculata predicted the possible amount of bioactive compound class like flavone, phenols, phenylpropanoids, flavonoids, phenolic acid, alkaloids, isopalmitic acid, diterpenoids and quinic acid. The best energy binding affinity score against sterol carrying protein-2 of A. aegypti, panicolin (-9.86 kcal/mol), berberine (-10.13 kcal/mol) and their combination (50:50) showed effective larvicidal movement with LC50 value of 25.41 ppm, 23.15 ppm and 17.56 ppm after 24 h treatment. Conclusion: The crude extracts, bioactive fractions and synthetic bioactive compounds and their combination study of A. paniculata and T. cordifolia can be utilized as bio-control agents against A. aegypti mosquito.

}, keywords = {Aedes aegypti mosquito larvae, Andrographis paniculata, Chromatographic techniques, In silico study, Larvicidal activity, Phytochemical screening, Tinospora cordifolia}, doi = {10.4103/pr.pr_35_20}, author = {Anubrata Paul and V Samuel Raj and Arpana Vibhuti and Ramendra Pati Pandey} }