@article {1164, title = {Formulation and in vitro Evaluation of Tablet Dosage form of Unani Anti-diabetic Powder Containing Gymnema sylvestre R. Br, Syzygium cuminii Linn. and Zingiber officinale Rosc.}, journal = {Pharmacognosy Research}, volume = {15}, year = {2023}, month = {June 2023}, pages = {566-577}, type = {Original Article }, chapter = {566}, abstract = {

Background and Objectives: Diabetes Mellitus (DM) is one of the leading causes of end stage renal disease, adult blindness etc. The side effects and resistance of conventional medicines for DM had put forward a need to develop an effective antidiabetic formulation with the aid of traditional medicine. Modification of dosage form in traditional system of medicine for palatability and portability is the need of hour. The antidiabetic safoof(powder) formulation containing Gurmar buti(Gymnema sylvestre R.Br), Jamun (Syzygium cuminii Linn.) and Zanjabeel (Zingiber officinale Rosc.) is having inconvenience to follow the accurate dose, palatability and stability issues due to surface area of powder. To rectify this problem the Safoof is modified into tablet dosage form with documentation of standard manufacturing procedure, its physicochemical analysis and in vitro antidiabetic evaluation. Materials and Methods: For process standardization total 10 batches were generated for the optimum working process related to the powder size, quantity of binder, wetting, granulation, time for drying and compression by trial and error. Ideal batch was selected on the basis of set parameter (friability, hardness and disintegration time), its physicochemical standards including HPLC as well as α-amylase and α-glucosidase inhibition activity were established. Results: With excipients 5\% Gum acacia, 5\% CMC, 1\% SSG, 0.5\% Mg stearate and 0.5\% Talc, the mean value of the hardness, friability and disintegration time of tablet were found to be 4.83 {\textpm} 0.17, 0.29 {\textpm} 0.01 and 14.24 {\textpm} 0.05 respectively. Physico-chemical data with HPLC quantification of Gymnemagenin, 6-Gingerol, 8-Gingerol, 6-Shogaol and 10-Gingerol were set in. The heavy metals and microbial contaminations are within permissible limit. Antidiabetic activity through α-amylase and α-glucosidase inhibition were 0.00 {\textpm} 0.00 at the 2500 μg/ml and 84.28 {\textpm} 0.58\% at the 3000 μg/ml concentration respectively. Conclusion: Classical powder is modified effectively in tablet dosage form with in vitro evaluation of its antidiabetic activity particularly α-glucosidase inhibition of crude powder and standardization data was also set in.

}, keywords = {Diabetes mellitus, Physico-chemical standardization, Powder, Tablets, α-glucosidase.}, doi = {10.5530/pres.15.3.060}, author = {Gazi Jahangeer Rather and Hamiduddin and Mohd Ikram and Md. Naquibuddin} } @article {55, title = {Cytotoxic and Antimigratory Effects on Michigan Cancer Foundation-7 Cells of Morinda citrifolia L. Leaf Extract and Formulation of Tablets from Extract}, journal = {Pharmacognosy Research}, volume = {12}, year = {2020}, month = {February 2020}, pages = {24-28}, type = {Original Article}, chapter = {24}, abstract = {

Background: Cancer is one of the deadliest diseases known to man. Efforts to combat the disease remain ongoing. Morinda citrifolia L. is a medicinal plant which is gaining interest as a natural chemotherapeutic agent for breast cancer treatment. Objective: This study aimed to determine the anticancer activity of M. citrifolia leaf extract on Michigan cancer foundation 7 (MCF-7) breast cancer cells as well as formulate tablets containing the extract to assess their viability. Materials and Methods: M. citrifolia leaf extract was prepared using the maceration method. Cytotoxicity and cell migration suppression, representing anticancer activity, were also assessed in MCF-7 cells by the sulforhodamine B assay and wound-healing assay. Tablets containing the extract were formulated using the wet granulation method. The prepared tablets were then evaluated regarding their physical properties and scopoletin content, a marker in the extract, before and after stability testing. Results: The extract showed cytotoxicity on MCF-7 cells in a dose- and time-dependent manner with 50\% inhibitory concentration values of 39.9 {\textpm} 3.5 {\textmu}g/mL for 48 h. In addition, it showed an antimigratory effect on MCF-7 cells with a significant effect at 25 {\textmu}g/mL. The prepared tablets had good characteristics and were found to meet the requirements of the United States Pharmacopeia. Moreover, they could maintain their physical properties and scopoletin content over a 2-month period. Conclusion: This study showed that M. citrifolia leaf extract exhibited potential anti-breast cancer activity. The prepared tablets containing the extract could be a potential formulation for breast cancer treatment. However, the underlying mechanism of the extract as an antibreast cancer agent requires further investigation in subsequent study.

}, keywords = {Antibreast cancer, Antimigratory effect, Morinda citrifolia L. leaf, Scopoletin content, Tablets}, doi = {10.4103/pr.pr_66_19}, author = {Supavadee Boontha and Benjaporn Buranrat and Tasana Pitaksuteepong} }