@article {281, title = {A Study of In-vitro Hypoglycemic and Glucose Uptake Activity of Isolated Compound from Ethanolic Leaf Extract of Amaranthus tristis Linn.}, journal = {Pharmacognosy Research}, volume = {11}, year = {2019}, month = {February,2019}, pages = {37-40}, type = {Original Article}, chapter = {37}, abstract = {

Background: Natural bioactive compounds have a long history to use effectively in the treatment of Diabetes Mellitus. Amaranthus tristis Linn (Amaranthaceae) have been used for the treatment of various ailments. Objective: The present work was undertaken to study the effect of an isolated bioactive compound from the leaf of Amaranthus tristis Linn on glucose uptake in cell lines like 3T3-L1 cell lines and inhibition of alphaglucosidase and alpha-amylase enzymes. Materials and Methods: The Ethanolic leaf extract of Amaranthus tristis Linn subjected to preliminary phytochemical screening and isolated compound were tested for its cytotoxicity study by MTT assay. Isolated compound was selected further glucose uptake assay based on cytotoxicity concentration CTC50 value. NMR technique was used to identify the structure of the compound. Results: Phytochemical analysis showed the presence of alkaloids, flavonoids, glycosides. The Isolated compound showed moderate cytotoxic activity to 3T3-L1 cell line. Compounds identified as rutin In in-vitro glucose uptake assay of rutin showed an increase in glucose uptake. Conclusion: A. tristis Linn. showed significant antidiabetic activity in dose-dependent manner.

}, keywords = {3T3-L1 cell lines, 3-(4, 5-dimethylthiazol-2-yl) 5-diphenyltetrazolium bromide assay, Amaranthus tristis Linn.}, doi = {10.4103/pr.pr_119_18}, author = {T. Sundarrajan and V. Velmurugan and K. Manikandan and D. Jothieswari} } @article {139, title = {A Study of In-vitro Hypoglycemic and Glucose Uptake Activity of Isolated Compound from Ethanolic Leaf Extract of Amaranthus tristis Linn}, journal = {Pharmacognosy Research}, volume = {11}, year = {2019}, month = {February 2019}, pages = {37-40}, type = {Original Article}, chapter = {37}, abstract = {

Background: Natural bioactive compounds have a long history to use effectively in the treatment of Diabetes Mellitus. Amaranthus tristis Linn (Amaranthaceae) have been used for the treatment of various ailments. Objective: The present work was undertaken to study the effect of an isolated bioactive compound from the leaf of Amaranthus tristis Linn on glucose uptake in cell lines like 3T3-L1 cell lines and inhibition of alphaglucosidase and alpha-amylase enzymes. Materials and Methods: The Ethanolic leaf extract of Amaranthus tristis Linn subjected to preliminary phytochemical screening and isolated compound were tested for its cytotoxicity study by MTT assay. Isolated compound was selected further glucose uptake assay based on cytotoxicity concentration CTC50 value. NMR technique was used to identify the structure of the compound. Results: Phytochemical analysis showed the presence of alkaloids, flavonoids, glycosides. The Isolated compound showed moderate cytotoxic activity to 3T3-L1 cell line. Compounds identified as rutin In in-vitro glucose uptake assay of rutin showed an increase in glucose uptake. Conclusion: A. tristis Linn. showed significant antidiabetic activity in dose-dependent manner.

}, keywords = {3T3-L1 cell lines, 3-(4, 5-dimethylthiazol-2-yl) 5-diphenyltetrazolium bromide assay, Amaranthus tristis Linn}, doi = {10.4103/pr.pr_119_18}, author = {T. Sundarrajan and V. Velmurugan and K. Manikandan and D. Jothieswari} } @article {345, title = {Study of Glucose Uptake Enhancing Potential of Fenugreek (Trigonella foenum graecum) Leaves Extract on 3T3 L1 Cells Line and Evaluation of its Antioxidant Potential}, journal = {Pharmacognosy Research}, volume = {10}, year = {2018}, month = {October 2018}, pages = {347-353}, type = {Original Article}, chapter = {347}, abstract = {

Background: The incidence of diabetes mellitus (DM) is increasing at an alarming rate globally. There is a need for suitable natural alternatives for its treatment, as the regular use of drugs causes several secondary health issues. Aim: This study aimed to quantitatively evaluate the phenol and flavonoid content; antioxidant and anti-diabetic activity of fenugreek seeds and leaves in water and ethanolic extracts. Materials and Methods: Dried and powdered seeds and leaves of fenugreek were treated at different time and temperature combinations (100{\textdegree}C for 10 min; 80{\textdegree}C for 30 min; 60{\textdegree}C for 1 h; 37{\textdegree}C for 6 h; 24{\textdegree}C [room temperature] for 24 h; 100{\textdegree}C for 10 min followed by overnight soaking). Total phenolic content, total flavonoid content, 2,2-Diphenyl-1-picrylhydrazyl (DPPH), and glucose uptake assays on 3T3-L1 cell lines were performed on the extracts. Results: The results show that fenugreek leaves treated at 100{\textdegree}C for 10 min and overnight soaking yield extracts with the highest concentrations of desired phenols (46.08 {\textpm} 0.15 mg GAE/g sample) and flavonoid content (13.02 {\textpm} 0.44 mg/g sample) and antioxidant activity (DPPH) (45.41 {\textpm} 2.1\%) with enhanced glucose uptake activity in 3T3-L1 cell lines. Conclusion: The phenolic and flavonoid content, DPPH radical scavenging activity and antidiabetic activity was highest in the water extract of fenugreek leaves treated at 100{\textdegree}C for 10 min and subsequent soaking for 24 h before filtration as compared to the other protocols tested. Furthermore, water extracts showed enhanced activity as compared to the ethanol extracts in case of both seeds and leaves and in all the treatment combinations.

}, keywords = {3T3-L1 cell lines, Antioxidant, Diabetes, Fenugreek, Glucose uptake}, doi = {10.4103/pr.pr_50_18}, author = {Debarupa Hajra and Santanu Paul} }