@article {1159, title = {Luffa operculata (L.) Cogn. Gestational Exposition Induces Anxiety-like Behavior and Interferes with Melatonin and Inflammation in Young Female Rats}, journal = {Pharmacognosy Research}, volume = {15}, year = {2023}, month = {June 2023}, pages = {524-536}, type = {Original Article }, chapter = {524}, abstract = {

Background: Luffa operculata (L.) Cogn. (Cucurbitaceae; EBN) dried fruits are abortifacient and showed undesirable effects over male and female adult Wistar rats. Objectives: The present study aimed at accessing how the gestational exposition of F1 female Wistar rats (FF1) to EBN interfered with behavior after challenge with stress or Lipopolysaccharide (LPS). Materials and Methods: Wistar rat female fetuses were gestationally exposed to a sub-abortifacient dose of EBN, and received stress or LPS challenge at a young adult age. The alterations in behavior, serum hormone levels, cytokines, and liver and kidney histological and biochemical indices were evaluated. Results: The gestational administration of EBN and NYM/LPS exposition at a young adult age induced anxiety-like behavior in the FF1, accompanied by an increase in the serum corticosterone, ACTH and melatonin levels. A diminish in the pro-inflammatory cytokines was also observed. No histochemical or biochemical alterations were seen in the liver or kidneys. Conclusion: The gestational exposition of EBN led the FF1 to reveal an anxiety-like behavior after being submitted to a stress challenge or LPS exposition at a young adult age and was accompanied by an increase of serum corticosterone and ACTH. A melatonin serum level increase has also been implicated in diminishing the pro-inflammatory cytokines.

}, keywords = {Animal behavior, Cognitive performance, Gestational treatment, Interleukins, Reproduction, Stress.}, doi = {10.5530/pres.15.3.055}, author = {Cinthia dos S. Alves and Humberto V. Frias and Leoni V. Bonamin and Eduardo F. Bondan and Cid{\'e}li P. Coelho and Maria de F{\'a}tima M. Martins and Maria M. Bernardi and Ivana B. Suffredini} } @article {138, title = {Effects of the Mint Monoterpene (R)-(+)-pulegone Evaluated by Functional Observational Battery: A Potential Short Method}, journal = {Pharmacognosy Research}, volume = {11}, year = {2019}, month = {February 2019}, pages = {31-36}, type = {Original Article}, chapter = {31}, abstract = {

Background: Pulegone (PUL) is one of the major constituents of peppermint and pennyroyal. Objective: The main purpose of this study was to investigate the early effects of toxicity of PUL. Materials and Methods: We assessed single doses of toxicity of PUL (2, 0.3 and 0.05 g/kg body weight), administrated by gavage in C57BL/6 mice, evaluated at 1, 6, and 24 h, their clinical status and behavioral, by functional observational battery (FOB), ambulatory conditions, sperm motility, pathological signs, and organ/body weight (O/Bw). Results: No mortality was registered in this in vivo study of oral acute toxicity, in which histological changes were found in selected organs, and PUL mainly showed that the highest concentration reduced mice locomotor activity with significant differences when compared with data of the FOB. Sperm motility also diminished, and hepatic as well as renal alterations were found without modifications in clinical status and O/Bw. Conclusions: We concluded that PUL could be responsible for these findings and consider that FOB is a useful tool to detect early signs of modifications of physiological and biological parameters in mice.

}, keywords = {Central nervous system, Functional observational battery, pulegone, Reproduction, Terpenes}, doi = {10.4103/pr.pr_115_18}, author = {Yesica P. Zaio and Marco M. Mazzotta and Agripina Ram{\'\i}rez S{\'a}nchez and Elisa Alejandrina Gomez and Mar{\'\i}a Paula Zunino and Andr{\'e}s Alberto Ponce} } @article {275, title = {Effects of the Mint Monoterpene (R)-(+)-Pulegone Evaluated by Functional Observational Battery: A Potential Short Method}, journal = {Pharmacognosy Research}, volume = {11}, year = {2019}, month = {February,2019}, pages = {31-36}, type = {Original Article}, chapter = {31}, abstract = {

Background: Pulegone (PUL) is one of the major constituents of peppermint and pennyroyal. Objective: The main purpose of this study was to investigate the early effects of toxicity of PUL. Materials and Methods: We assessed single doses of toxicity of PUL (2, 0.3 and 0.05 g/kg body weight), administrated by gavage in C57BL/6 mice, evaluated at 1, 6, and 24 hr, their clinical status and behavioral, by functional observational battery (FOB), ambulatory conditions, sperm motility, pathological signs, and organ/body weight (O/Bw). Results: No mortality was registered in this in vivo study of oral acute toxicity, in which histological changes were found in selected organs, and PUL mainly showed that the highest concentration reduced mice locomotor activity with significant differences when compared with data of the FOB. Sperm motility also diminished, and hepatic as well as renal alterations were found without modifications in clinical status and O/Bw. Conclusion: We concluded that PUL could be responsible for these findings and consider that FOB is a useful tool to detect early signs of modifications of physiological and biological parameters in mice.

}, keywords = {Central nervous system, Functional observational battery, pulegone, Reproduction, Terpenes.}, doi = {10.4103/pr.pr_115_18}, author = {esica P. Zaio and Marco M. Mazzotta and Agripina Ram{\'\i}rez S{\'a}nchez and Elisa Alejandrina Gomez and Mar{\'\i}a Paula Zunino and Andr{\'e}s Alberto Ponce} }