@article {1397, title = {Bioactive Compounds in Inocutis levis and their Cytotoxic Effects on MCF-7 and HT-29 Cancer Cells}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {April 2025}, pages = {1-11}, type = {Original Article}, chapter = {1}, abstract = {

Background and Objectives: Fungi are undeniable sources of all types of secondary metabolites and bioactive compounds. Inocutis levis is a polypore fungus belonging to Hymenochaetales of basidiomycetes. This study aimed to identify the compounds in the extract and investigate the cytotoxicity effect of Inocutis levis on different cell lines (MCF-7, HT-29 and HFF). Materials and Methods: The extract was obtained by rotary evaporator and LC/MS was used to identify the compounds in the extract. The cytotoxic effect of Inocutis levis against MCF-7, HT-29 and HFF cells was evaluated using MTT assay, 24 and 48 hr after the addition of the extract. Also, flow cytometry analysis was carried out to study the cell cycle arrest and apoptosis.Results: The LC/ MS assay data showed that the mushroom extract contains antioxidant compounds. Based on the MTT test, Inocutis levis extract did not decrease the viability of non-cancerous HFF cells. Still, it was able to inhibit the growth and increase the death in HT-29 cells at a concentration of 100 μg/mL after 24 and 48 hr (p\<0.05) also, MCF-7 cells at concentrations of 1 and 10 and 100 μg/ mL after 24 and 48 hr (p\<0.05). Evaluation of the cell cycle 48 hr after adding the extract by flow cytometry, showed that the mushroom extract induced apoptosis in MCF-7 cells. Conclusion: Inocutis levis contains antioxidant compounds and can inhibit the growth of HT-29 and especially MCF-7 cancer cells via the induction of apoptosis.

}, keywords = {anticancer, Apoptosis, Cell cycle, HT-29, Inocutis levis, LC/MS, MCF-7}, doi = {10.5530/pres.20252069}, author = {Fereshteh Mir Mohammad Rezaei and Fatemeh Tavakoli and Maryam Kiaei and Vahid Ramezani and Amin Zarezadeh and Masoumeh Ghobad-Nejhad} } @article {1281, title = {Screening of in vitro Anticancer Activity of Various Human Cancer Cell Lines and Induced Apoptosis and Cell Arrest by Ethanolic Bulb Extract of Pancratium zeylanicum}, journal = {Pharmacognosy Research}, volume = {16}, year = {2024}, month = {October 2024}, pages = {718-726}, type = {Original Article}, chapter = {1}, abstract = {

Aim: World widely the human society suffers from various types of cancer due to many causes. Thus, finding out a good anticancer agent is a need of the hour. Medicinal herbs are always considered at pole position whenever we discuss anticancer properties. Numerous herbal plants have been assessed and are subjected to phytochemical analysis to learn more about their anticancer effects. Materials and Methods: To assess the anti-cancerous activity of PZBE extract in MTT assay, cell cycle analysis, and apoptosis studies have been performed using different cancer cell lines. Results: This study aims to assess the impact of ethanolic extract of Pancratium zeylanicum Bulbs (PZBE) against cancerous cells in vitro conditions. MTT assay was used to compare the PZBE extract with Annexin V as a positive control compound against four human cancer cell lines, including Human cervical cancer (HeLa), colon cancer (HT-29), lung cancer (A549) and breast cancer (MCF-7) cell lines with the IC50 values of 209.28 μg/mL for HeLa, 133.09 μg/mL for A-549, 264.46 μg/mL for MCF-7 and 264.75 μg/mL for HT-29 cell lines. Increasing of the populations observed in G2-M and G0-G1 cells by 42\% and 44.78\%, respectively when compared to the control. The graph demonstrates that PZBE extracts significantly induced G0-G1 arrest in A-549 cells. Furthermore, the PZBE extract effectively induced apoptosis when compared to untreated cells, A-549 cells treated with IC50 concentration, and annexin-V double-staining assay demonstrated an increase in the percentage of early and late apoptotic cells. Conclusion: The ethanol extract of Pancratium zeylanicum bulbs demonstrated potent cytotoxic activity against the cancer cell lines HeLa, A-549, MCF-7, and HT-29. The current study{\textquoteright}s findings may be helpful in the development of plant-based anticancer drugs.

}, keywords = {A-549, Annexin-V, Anticancer activity, Apoptosis, Ethanol extract, HeLa, HT-29, MCF-7}, doi = {10.5530/pres.16.4.83}, author = {Elumalai Shanthi Priya and Devaraj Sathiya Sheela and Devaraj Sheeba Gnanadeebam and Balrajmani Davidson Raja and Periannan Viswanathan} }