@article {1420, title = {Isolation, Characterization and Structural Elucidation of a Bioactive Flavonoid from Schinus polygama Leaves Using Chromatographic and Spectroscopic Techniques}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {April 2025}, pages = {627-637}, type = {Original Article}, chapter = {627}, abstract = {

Aim: Isolation, Characterization and Structural Elucidation of a Bioactive Flavonoid from Schinus polygama Leaves Using Chromatographic and Spectroscopic Techniques is the aim of present study. The process of separating and characterizing bioactive substances from natural sources are pivotal in understanding their therapeutic potential. Materials and Methods: In the present study, Thin-Layer Chromatography (TLC) with optimized solvent system used. Further this solvent system was employed in column chromatography to isolate active fractions. Active fraction characterized by UV-visible spectroscopy, FT-IR analysis, Nuclear Magnetic Resonance (NMR) and mass spectroscopy. Results: Schinus polygama (SP) (family Anacardiaceae) extract was subjected to Thin-Layer Chromatography (TLC) using various solvent systems, with the optimized system of Acetic Acid: Ethyl acetate: Toluene (0.4:4:6) yielding a distinct band with 0.64 Rf value, corresponding to a standard flavonoid. This solvent system was employed in column chromatography, leading to the separation of multiple fractions, including the active Fraction (F). UV-visible spectroscopy of Fraction (F) showed absorption peaks at 208, 269 and 365 nm, indicating conjugated π-electron systems. The presence of distinctive functional groups was further verified by FT-IR analysis., including hydroxyl (-OH), alkyl (C-H) and aromatic (C=C) bonds. Nuclear Magnetic Resonance (NMR) spectra revealed specific proton signals consistent with a flavonoid structure, while mass spectrometry identified a molecular ion peak which corresponds to 3,5,7-trihydroxy-2-(4-hydroxyphenyl) chromen-4-one at m/z 286.20 with a molecular formula of C15H10O6. Conclusion: These findings suggest that the isolated compound from Schinus polygama leaves ethanolic extract is a flavonoid with potential biological activities. Further studies are recommended to explore its pharmacological properties and potential applications in therapeutic formulations

}, keywords = {Active fraction, Characterization, Flavonoid, Isolation, Spectroscopy}, doi = {10.5530/pres.20252098}, author = {Chandra Prakash Bhardwaj and Piush Sharma and Maneesh Napit} } @article {1259, title = {LC-MS and HPTLC Profile of Crude Ethanol Extract of Plant Michelia champaca Linn.}, journal = {Pharmacognosy Research}, volume = {16}, year = {2024}, month = {June 2024}, pages = {490-494}, type = {Original Article}, chapter = {490}, abstract = {

Aim: The plant Michelia champaca L. plant is a very rich source of phytoconstituents with medicinal properties. The aim of the study is to analyze the chemical constituents present in the crude extract responsible for various pharmacological actions. Materials and Methods: The aerial parts of the plant Extracted with ethanol and subjected to LC-MS and HPTLC analysis by using Water Q-TOF micro coupled with 2795 HPLC and CAMAG TLC SCANNER -3. Results: LC-MS analysis showed the various peaks with low and high molecular weights indicating the presence of various phytochemicals. The compounds identified in the ethanol extract include parthenolides, quercetin, stigmasterol, sitosterol, and liriodenine and some unknown compounds by comparison with the SDBS database. HPTLC of the ethanol extract showed the Rf value of five compounds, were found to be more prominent. 0.15, 0.3, 0.45, 0.6, 0.75. identified by comparison with HPTLC - derived database. Conclusion: The compounds identified by LC-MS and HPTLC of the ethanol extract of the aerial plant Michelia champaca Linn. may be responsible for its various pharmacological activities, and hence this plant can be used in the treatment of various ailments.

}, keywords = {Chromatography, Extract, Plant, Spectroscopy}, doi = {10.5530/pres.16.3.59}, author = {Chander Mohan and Seema Devi} }