@article {1463, title = {In vitro and Computational Studies of Compounds from Coleus amboinicus against Osteosarcoma}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {June 2025}, pages = {997-1004}, type = {Original Article}, chapter = {997}, abstract = {

Background: Osteosarcoma (OS) is among the most prevalent primary bone cancers, predominantly affecting children and adolescents. The average prevalent rate of osteosarcoma is 4.3 per million in males and 3.4 per million in females. Osteosarcoma poses diagnostic challenges due to the often-subtle clinical manifestations. Objectives: To investigate bioactive compounds in Coleus amboinicus Leaves (CAL) using computational methods and predict, their potential interactions with osteosarcoma cell surface receptors to explore therapeutic possibilities. Materials and Methods: The extract obtained from CAL was analyzed using a phytochemical assay, Fourier-Transform Infrared (FTIR) spectroscopy, and Gas Chromatography-Mass Spectrometry (GCMS). Molecular docking studies were conducted using Autodock to assess the interactions between the components of the extract and the cell surface receptors of osteosarcoma. The molecular docking experiments assessed the affinity of the phytochemicals to particular proteins that have a significant impact on the cell surface receptors of osteosarcoma. The pharmacokinetic characteristics of the drugs were assessed by examining their ADMET (absorption, distribution, metabolism, excretion, and toxicity) profiles. Results: The computer study identified multiple phytochemicals derived from CAL with substantial binding affinity to the proteins of osteosarcoma cell surface receptors. These substances include beta-D-Glucopyranose, 4-O-beta-D-galactopyranosyl, Hexanoic acid, 1-methylethyl ester, and Paromomycin. The ADMET analysis revealed that most of the discovered drugs possess favorable pharmacokinetic features and are anticipated to have low toxicity. Conclusion: Molecular docking revealed bioactive compounds with strong binding affinity to these receptors, indicating their potential inhibitory effects on cancer cell proliferation. Further research is required to provide comprehensive validation and clinical development.

}, keywords = {Bioactive compounds, GCMS, Molecular docking, Osteosarcoma, Phytochemical test, Plant extract}, doi = {10.5530/pres.20252178}, author = {Archana Behera and Raeesha Rahman and Benjamin Vinodh Joshua and Iadalin Ryntathiang and Monisha Prasad and Mukesh Kumar Dharmalingam Jothinathan} } @article {1215, title = {Evaluation of the Effect of Five Colombian Propolis Extracts on the Expression of Genes Associated with Cell Cycle and Inflammation in a Canine Osteosarcoma Cell Line}, journal = {Pharmacognosy Research}, volume = {16}, year = {2023}, month = {December 2023}, pages = {172-182}, type = {Original Article}, chapter = {172}, abstract = {

Introduction: Propolis has anti-inflammatory, antitumor, antibacterial and immunomodulatory properties, which is why it is suggested that it could be used as an alternative or complementary drug therapy in the treatment of various pathologies such as cancer, diseases chronic-degenerative and infectious. Materials and Methods: In this study, canine bone fibroblasts were used as control cells, and the canine Osteosarcoma cell line (OSCA-8) was acquired to evaluate the effects of ethanol extracts of propolis from five Colombian regions on these cells. The cytotoxic effect was evaluated by examining changes in cell viability and proliferation. Furthermore, the expression of some relevant and characteristic genes related to the tumor phenotype, related to the proinflammatory process and cell cycle, was assessed. Results: Thus, the evaluation of the relative expression of some genes associated with the cell cycle and inflammation could improve the understanding of the cytotoxic effect of propolis extracts on OSCA-8 cell lines. Conclusion: The first time in Colombia, the biological activity of ethanol extracts of propolis was evaluated regarding the inflammation and cell cycle pathways. After 48 hr, the Colombian EEP had an effect on the increase in both OSCA-8 cells and fibroblasts.

}, keywords = {Biological activity, Cell cycle, Gene expression, Inflammation, Osteosarcoma, Propolis.}, doi = {10.5530/pres.16.1.22}, author = {Oscar Juli{\'a}n Murillo Torres and Dolly Patricia Pardo Mora and Orlando Torres Garc{\'\i}a and Mauricio Rey Buitrago} }