@article {1265, title = {Extraction, Quantification and Anti-Cancer Activity of Important Secondary Metabolite {\textquotedblleft}Shatavarin-IV{\textquotedblright} from in vitro Callus Culture of Asparagus racemosus Willd.}, journal = {Pharmacognosy Research}, volume = {16}, year = {2024}, month = {June 2024}, pages = {558-569}, type = {Original Article}, chapter = {558}, abstract = {

Objectives: This study investigates the in vitro efficient pathway for the callus induction from nodal explant and isolation of the Shatavarin-IV from the callus extract from fresh callus and Dry callus. The effect of Shatavarin IV was examined on the Human lung carcinoma cell line. Gene expression study was carried out using a specific primer to conform weather it is anticancer compound or not. Materials and Methods: In the present study, 2,4-D and Kn were used for the direct callus induction. Callus culture, organogenesis and cell culture are used to harvest secondary metabolites. Quantification of Shatavarin-IV was performed using a TLC scanner at a wavelength of 425 nm. HPTLC analysis was performed to quantitatively determine the presence of Shatavarin-IV in Fresh Callus (FC), Dry Callus (DC) and Root Extract (RE). The mobile phase used for HPTLC analysis was ethyl acetate: methanol: water: formic acid (7.5:1.5:1:0.2 v/v). Extraction was carried out using 80\% methanol and the extract was filtered. The extract was then analyzed using HPTLC methods. Further, the effect of Shatavarin-IV was studied on the human lung carcinoma cell line NCI-H23. The end result of cell cytotoxicity was analyzed using MTT dye. To, confirm whether the cell expressed an apoptotic gene or not RTPCR studies were been carried out. Results: The best hormonal combination for callus induction from nodal explant was found 11.31 μM 2,4-D combined with 4.64 μM Kn on MS as basal media. Shatavarin-IV accumulation in dry callus was 0.0305\% w/w, wild-grown plant root extract showed 0.01732\% w/w and in fresh callus presence of Shatavarin-IV was 0.0012\% w/w. IC50 of the Shatavarin-IV was 0.8 Micromolar dissolved in DMSO. Gene expression study reflects the multifold increase of the BAX and decrease of BCL2, concluded interpreted as Shatavarin-IV causes apoptotic gene expression. Conclusion: It was concluded that, Shatavarin IV reduced the expression of BCL2 and increase BAX expression which positive sign that Shatavarin IV act as an anticancer compound. Shatavarin IV can be isolated from the in vitro callus culture, which can be alternative of whole plant that was exploited for its medicinal properties. (Schematic Abstract).

}, keywords = {Antiproliferative, Dry Callus, Fresh Callus, Gene expression, Phylloclade, Shatavarin-IV}, doi = {10.5530/pres.16.3.66}, author = {Dinesh Vasava and Poonam Bhagriya, Abhishek Tote and Keyur Sindhava, Nitin Solanki} } @article {1215, title = {Evaluation of the Effect of Five Colombian Propolis Extracts on the Expression of Genes Associated with Cell Cycle and Inflammation in a Canine Osteosarcoma Cell Line}, journal = {Pharmacognosy Research}, volume = {16}, year = {2023}, month = {December 2023}, pages = {172-182}, type = {Original Article}, chapter = {172}, abstract = {

Introduction: Propolis has anti-inflammatory, antitumor, antibacterial and immunomodulatory properties, which is why it is suggested that it could be used as an alternative or complementary drug therapy in the treatment of various pathologies such as cancer, diseases chronic-degenerative and infectious. Materials and Methods: In this study, canine bone fibroblasts were used as control cells, and the canine Osteosarcoma cell line (OSCA-8) was acquired to evaluate the effects of ethanol extracts of propolis from five Colombian regions on these cells. The cytotoxic effect was evaluated by examining changes in cell viability and proliferation. Furthermore, the expression of some relevant and characteristic genes related to the tumor phenotype, related to the proinflammatory process and cell cycle, was assessed. Results: Thus, the evaluation of the relative expression of some genes associated with the cell cycle and inflammation could improve the understanding of the cytotoxic effect of propolis extracts on OSCA-8 cell lines. Conclusion: The first time in Colombia, the biological activity of ethanol extracts of propolis was evaluated regarding the inflammation and cell cycle pathways. After 48 hr, the Colombian EEP had an effect on the increase in both OSCA-8 cells and fibroblasts.

}, keywords = {Biological activity, Cell cycle, Gene expression, Inflammation, Osteosarcoma, Propolis.}, doi = {10.5530/pres.16.1.22}, author = {Oscar Juli{\'a}n Murillo Torres and Dolly Patricia Pardo Mora and Orlando Torres Garc{\'\i}a and Mauricio Rey Buitrago} }