@article {1209, title = {Elucidation of the Anti-inflammatory, Anti-proliferative and Epithelial Mesenchymal Transition Inhibiting Potentials of Cichorium intybus Extract on Human Cancer Cell Line(s)}, journal = {Pharmacognosy Research}, volume = {16}, year = {2023}, month = {December 2023}, pages = {132-139}, type = {Original Article}, chapter = {132}, abstract = {

Background: Cichorium intybus L. is cultivated in various temperate regions of the world for use of its different parts (mostly roots/seeds) as medicines or food. However, the anti-inflammatory, anti-proliferative and Epithelial Mesenchymal Transition (EMT)-inhibiting potential of C. intybus extracts have not been extensively studied at molecular level using suitable human cancer cell-line models. Objectives: Elucidation of the anti-inflammatory, anti-proliferative and EMT inhibiting potential of C. intybus extract using human cancer cell-line models. Materials and Methods: We prepared Methanolic Extract (MKE) of C. intybus leaves and studied its anti-inflammatory, anti-proliferative, and anti-EMT activities in THP-1 and U87MG cell lines through real time expression analysis and various functional assays including cell viability, cytotoxicity, colony forming and wound healing assays. Results: MKE significantly reduced the LPS-induced transcription of proinflammatory cytokines; TNF-α, and IL-6 from macrophage turned THP-1 cells. Also, expression of TGF-β gene was significantly downregulated by MKE in both THP-1 macrophages and U87MG cells. In U87MG glioma cells, MKE significantly downregulated the expressions of proliferation (Ki67, and PCNA), and EMT markers (LIX1, VIM, ZEB1, SNAIL, and SLUG), which are associated with aggressiveness, histological grade and poor prognosis in glioma. Conclusion: It should be emphasized that this is the first study done on examining the potential of C. intybus anti-inflammatory, anti-proliferative, and anti-EMT activities against the THP-1 macrophages and U87MG glioblastoma cells. Our findings strongly suggest C. intybus leaves as an efficient source of pharmaceutical formulations that can contribute to the treatment of inflammatory diseases and cancer and could add in improving human health significantly.

}, keywords = {Anti-EMT, Anti-inflammatory, Anti-neoplastic, Anti-proliferative, Cichorium intybus}, doi = {10.5530/pres.16.1.16}, author = {Ankita Harishchandra Tripathi and Amrita Kumari and Rahul Anand and Ramesh Chandra Rai and Poonam Gautam and Penny Joshi and Santosh Kumar Upadhyay} } @article {1147, title = {Insight on Phytochemical and Pharmacognostic Review of Cichorium intybus}, journal = {Pharmacognosy Research}, volume = {15}, year = {2023}, month = {June 2023}, pages = {405-409}, type = {Review Article}, chapter = {xx}, abstract = {

In today{\textquoteright}s growing world herbs are gaining a lot of importance because of their ability to improve human health. One such herb is Cichorium intybus. It is also called as chicory/kasni representing Asteraceae family and cultivated across various parts of the globe. Cichorium intybus grows up to a height of 150 cm having thick, strong, irregularly branched twig like stem, long robust thick brown tapio root, lobed and unlobed leaves, seed like fruits and flowers of various colours. Chicory contains various chemical constituents like cichoric acid, sesquiterpenes, lactones, caffeic acid derivatives, inulin, sugars, proteins, sugars, volatile compounds and many more. The phytochemical composition of chicory exhibits not only various pharmacological activities such as nematicidal, hypotensive, analgesic and hepatoprotective but also used in the treatment of several diseases like diabetes, cancer, viral infections, bacterial infections, allergic reactions and antioxidant property respectively. It also has great importance in the field of Ayurveda, it had various medicinal properties and used as a flavouring agent in different food preparations like soups and salads. Current review aimed to emphasize the botanical characteristics, chemical composition, pharmacological activities and culinary applications of Cichorium intybus.

}, keywords = {Chemical composition, Cichorium intybus, Culinary applications, Pharmacological activity}, doi = {10.5530/pres.15.3.044}, author = {Nimmathota Madhavi and B. Lohith Chandu and G. Rithika and B. Niharika and T. Rama Rao} }