@article {1181, title = {Estimation of Ursolic Acid in Methanolic Extract of Momordica dioica by HPTLC Method}, journal = {Pharmacognosy Research}, volume = {15}, year = {2023}, month = {October 2023}, pages = {727-732}, type = {Original Article}, chapter = {727}, abstract = {

Background: A number of nonsteroidal anti-inflammatory medications have been shown to lessen pain and inflammation. Unfortunately, there are numerous side effects connected with their use. There are, however, medicinal plants that have anti-inflammatory therapeutic effects with little to no side effects. Momordica dioica is a herb that belongs to the Cucurbitaceae family. There are numerous phytoconstituents in it. Ursolic acid is one of them and a pentacyclic terpenoid that is found in nature and has a number of therapeutic benefits. The current study looked at Ursolic Acid{\textquoteright}s activities (UA), a secondary plant metabolite, for its anti-inflammatory properties in the Momordica dioica plant. The current study{\textquoteright}s objective was to create and validate an HPTLC method that was quick, accurate, precise, and specific for determining ursolic acid from Momordica dioica herbal extract. Methodology/Conclusion/Significance: For quick analysis of Ursolic acid determination, The High Performance Thin Layer Chromatography (HPTLC) was established and confirmed. On an aluminium HPTLC plate (60 F254) coated with precoated silica gel with formic acid, ethyl acetate, and toluene (7:3:0,1), chromatographic separation was accomplished. The detection process was carried out at 530 nm. Ursolic acid{\textquoteright}s Rf value was discovered to be 0.795\%. In the 400ng/band concentration range, linearity for ursolic acid was detected. The limits of detection and quantitation for ursolic acid were observed to be 0.04 ng/ band and 0.14 ng/band, respectively. The mean \% recovery of rosmarinic acid was (0.54). The method{\textquoteright}s specificity, robustness, linearity, precision, and accuracy have all been validated in compliance with ICH standards. The created method can be used to evaluate the regularity of ursolic acid in herbal extracts.

}, keywords = {Chromatography, Herbal extract, HPTLC, Inflammation, Methanol, momordica dioica, Rf value, Ursolic acid}, doi = {10.5530/pres.15.4.077}, author = {Jui Darbhe and Vrushali Neve and Vrushali Bhalchim and Ashwini Tonpe and Pritam Khandave and Suchita Mane and Rupali Yewale} } @article {1128, title = {Preparation of Camellia sinensis (Green Tea) and Acacia nilotica (Babul) Herbal Formulation and its Anti-Inflammatory Activity}, journal = {Pharmacognosy Research}, volume = {15}, year = {2023}, month = {February 2023}, pages = {255-257}, type = {Original Article }, chapter = {255}, abstract = {

Introduction: Acacia nilotica is otherwise called Babul is multipurpose nitrogen fixing tree legume. Its roots are beneficial in treating tumors, cancer, and tuberculosis. One of the most consumed beverages in the world, green tea (Camellia sinensis) has been researched for its potential health benefits in treating conditions like cancer, diabetes, obesity, heart disease, and neurological illnesses. The aim of this present study is to assess the combined effects of green tea and babul herbal formulation as an anti-inflammatory agent. Materials and Methods: Green tea and Babul extract was prepared. Albumin denaturation assay and egg albumin denaturation assay were used to assess the anti-inflammatory activity. Results: Both assays showed that Green tea and babul extract was effective in inhibiting the albumin denaturation at different concentrations. Conclusion: We conclude that green tea and babul herbal formulation can be a potent anti-inflammatory agent.

}, keywords = {Anti-inflammatory activity., Babul, Green tea, Herbal extract}, doi = {10.5530/pres.15.2.027}, author = {Amirtha Varshini and Anju Cecil and S. Rajesh Kumar} }