@article {1348, title = {GC-MS Analysis of Bioactive Compounds Present in the Different Extracts of the Whole Plant Impatiens minor (DC.) Bennet}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {January 2025}, pages = {126-132}, type = {Original Article}, chapter = {126}, abstract = {

Background and Objectives: The systematic assessment of herbs utilized in the traditional medicine could provide more promising data regarding their therapeutic properties helping to address the increasing need for innovative treatments for infections and illnesses. Considering this perspective, Impatiens minor, an important medicinal plant distributed in the wet wastelands throughout India and also in the wet forests of Western Ghats was selected for our research. Materials and Methods: In this study, the plant I. minor in whole was collected, authenticated and subjected to shade drying for powdering which was done by mechanical grinder. Soxhlation technique was employed for the extraction of powdered plant material using different solvents to prepare the dried extract which was subjected to preliminary phytochemical evaluation and GC-MS analysis. Results: Presence of glycosides, phenolic compounds, flavonoids, terpenoids, sterols and carbohydrates was found in the preliminary phytochemical evaluation of different extracts. In the GC-MS analysis, presence of eight major compounds were found in the petroleum ether extract, while the chloroform extract showed the presence of six major compounds and twelve major compounds were found in the ethanol extract. Conclusion: It is worth noting that certain compounds identified in the GC-MS analysis have been documented to possess anti-oxidant, anti-inflammatory, and anticancer properties in previous studies. This study has the potential to contribute valuable insights for future in-depth investigations.

}, keywords = {Extraction, GC-MS Analysis, Impatiens minor (DC.) Bennet, Preliminary phytochemical evaluation}, doi = {10.5530/pres.20251952}, author = {Akila Sukumaran and Ansa Mathew and Sebastin Varghese and Ajithbabu Tharackal Kannankutty} } @article {1089, title = {In vitro Antioxidant Activity and Preliminary Phytochemical Evaluation of Different Extracts of Aerva javanica}, journal = {Pharmacognosy Research}, volume = {14}, year = {2022}, month = {October 2022}, pages = {468-473}, type = {Original Article}, chapter = {468}, abstract = {

Background: Ethno medicinal validation, together with the screening of phytochemical and biological activity, is an effective method for identifying new drugs from medicinal herbs. Objectives: The present study was aimed to investigate the phytochemical nature and antioxidant potential of the selected plant Aerva javanica. Materials and Methods: The plant material of A. javanica was collected, authenticated, leaf and stem parts were separated, shade dried, powdered separately in to coarse powder and extracted with ethanol by Soxhlet apparatus. Both extracts (leaves and stem) were subjected to phytochemical evaluation, total phenolic content estimation by Folin-Ciocalteau and the total flavonoids content estimation by using aluminium chloride colorimetric method. In vitro antioxidant evaluation of the leaves and stem extracts were done by different approaches such as DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay, reducing power assay, superoxide and nitric oxide radical scavenging assays. Results: In the preliminary phytochemical analysis, both leaves and stem extract showed the presence of alkaloids, glycosides, tannins and phenolic compounds and flavonoids. The results showed the absence of carbohydrates, proteins and amino acids and sterols. The total phenolic content in the ethanol extract of A. javanica leaves and stems were found to be 891mg/g and 841mg/g of extract equivalent to gallic acid. The total flavonoids content in the ethanol extract of A. javanica leaves and stems were found to be 950mg/g and 900mg/g of extract equivalent to quercetin. The leaves extract showed maximum antioxidant activity comparing with stem extract in all the test approaches employed. Conclusion: The chromatographic and spectral studies and detailed pharmacological evaluation of the extracts may give more promising data.

}, keywords = {Aerva javanica, Antioxidant activity, Preliminary phytochemical evaluation, Total flavonoid estimation, Total phenolic estimation}, doi = {10.5530/pres.14.4.68}, author = {CK Thasneem and GR Vijayasankar and BS Venkateswarlu and R Margret Chandira and S Shanmuganathan} }