@article {116, title = {Safety and Antioxidant Potential of Traditional Thai Poly-Herbal Tea {\textquotedblleft}Phy-Blica-D{\textquotedblright} Used as a Rejuvenation Formula}, journal = {Pharmacognosy Research}, volume = {11}, year = {2019}, month = {August 2019}, pages = {295-303}, type = {Original Article}, chapter = {295}, abstract = {

Background: The rising popularity of phytonutrient consumption may be due to a vast number of scientific studies that have revealed their health benefits; however, concerns regarding the medical safety of herbal-based products are increasing. Phy-Blica-O is Phyllanthus emblica-based herbal tea used in Thai traditional medicine as a rejuvenating remedy. However, its consumption has been limited due to its strong bitter taste with unpleasant odor. Objective: The objective of this study is to evaluate the safety and antioxidant potential of Phy-Blica-D, the modified formula of Phy-Blica-O which gave high sensory acceptability scores. Materials and Methods: Subacute toxicity studies of Phy-Blica-D infusion was conducted by repeated oral administration of the extract at doses of 5, 50, and 300 mg/kg/day in Sprague-Dawley rats. Results: The formula exhibited antioxidant activity with an IC50 of 0.243 {\textpm} 0.006, 0.486 {\textpm} 0.002 and 0.108 {\textpm} 0.004 mg/mL using 1,1-diphenyl-2-picrylhydrazyl, 2,20-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid and metal chelating assays, respectively. There was no significant treatment-related toxicity as indicated by clinical signs, body weight, food consumption, serum biochemical and hematological parameters, organ weight, and histopathological examination of the animals treated with Phy-Blica-D infusion. These results suggest that the oral no-observed-adverse-effectlevel of Phy-Blica-D extract is \>300 mg/kg body weight/day, or ~3.7 L/kg body weight/day for both sexes. The calculated human equivalent dose value is 48.39 mg/kg/day, or ~600 mL/kg body weight/day. There were no target organs affected. Conclusion: This study demonstrates that Phy-Blica-D infusion can be regarded as safe and could potentially be used as a functional ingredient to reduce oxidative stress in non-communicable diseases.

}, keywords = {Antioxidant activity, Folkloric infusion, Functional beverage, Phyllanthus emblica, Subacute toxicity study.}, doi = {10.4103/pr.pr_5_19}, author = {Acharaporn Issuriya and Nuntitporn Puangkeaw and Piyapong Choochana and Patcharawalai Jaisamut and Nongluk Kunworarath and Katesarin Maneenoon and Surasak Limsuwan and Thammarat Kaewmanee and Sasitorn Chusri} } @article {697, title = {Evaluation of Phyllanthus emblica Extract on Cold Pressor Induced Cardiovascular Changes in Healthy Human Subjects}, journal = {Pharmacognosy Research}, volume = {6}, year = {2014}, month = {December,2013}, pages = { 29-35}, type = {Original Article}, chapter = { 29}, abstract = {

Background: Acute and chronic stress is a risk factor for the development and progression of coronary artery disease. Increased arterial stiffness is an independent marker for cardiovascular disease. Cold pressor test (CPT) is known to be associated with substantial activation of the autonomic nervous system. Objective: The aim of this study was to evaluate the effect of Phyllanthus emblica extract on cold pressor stress test induced changes on cardiovascular parameters and aortic wave reflections in healthy human subjects. Materials and Methods: This was a double-blind, placebo-controlled, crossover study. Participants were randomized to receive either two capsules of P. emblica extract 250 mg (containing aqueous extract of P. emblica, highly standardized by high-performance liquid chromatography to contain low molecular weight hydrolysable tannins emblicanin-A, emblicanin-B, pedunculagin and punigluconin) or two capsules of placebo twice daily for 14 days. Pharmacodynamic parameters such as heart rate, augmentation pressure, augmentation index (AIx), subendocardial viability ratio (SEVR), radial and aortic blood pressure (BP) were recorded before and after CPT at baseline and end of treatment. After washout period of 14 days, subjects crossed over to the other treatment and the same test procedure was repeated again. Safety assessments were done at baseline and at the end of treatment. Results: A total of 12volunteers completed the study. Compared with baseline and placebo, P. emblica extract produced a significant decrease of mean percent change in the indices of arterial stiffness (AIx, radial and aortic BP) and increase in SEVR, an index of myocardial perfusion with CPT. Both treatments were well-tolerated and no serious adverse events were reported. Conclusion: Proprietary P. emblica extract, showed a significant decrease in cold pressor stress test induced changes on aortic wave reflections.

}, keywords = {Augmentation index, Cardiovascular disease, Cold pressor test, Phyllanthus emblica}, doi = {10.4103/0974-8490.122914}, author = {Nishat Fatima and Usharani Pingali and Raveendranadh Pilli} }