@article {1129, title = {Abrogation of Acute Inflammation in ICR Mice with Topical Administration of Philippine Stingless Bee (Tetragonula biroi Friese) Honey}, journal = {Pharmacognosy Research}, volume = {15}, year = {2023}, month = {February 2023}, pages = {258-266}, type = {Original Article }, chapter = {258}, abstract = {

Background: Honey, a sweet sugar-rich bee-derived product, has been widely recognized in the realm of Ayurveda and Pharmacognosy for the treatment of various inflammatory conditions including inflammatory bowel disease, asthma, arthritis, and gastric ulcer. However, the anti-inflammatory effect of Philippine honey obtained from local stingless bees remains unexplored and poorly investigated. Hence, this study delved on the anti-inflammatory potential of topically applied Philippine stingless bee honey (PSH) using the λ-Carrageenan-induced mouse model of paw edema. Materials and Methods: A total of 30, male ICR mice of six weeks of age were randomly divided into the following groups (n=10 per group) as follows: Distilled water-, 1\% Diclofenac sodium-, and Honey-treated group. All treatments were administered immediately after Carrageenan injection and then every 8 hr during the 24-hr test duration. Results: Repeated topical application of PSH significantly attenuated the gross hind paw swelling observed at the onset of the 6 hr post-induction (pi) until the 24 hr pi with Carrageenan in contrast to the Distilled water-treated group. This corresponded histologically to a marked reduction in dermal thickness, dermal edema, and leukocytic infiltration. Whereas, at the cytokine level, this correlated to significant suppression of the circulating blood levels of pro-inflammatory cytokines, TNF-α, IL-1β, and IL-6. Conclusion: Based on these results, honey from the Philippine stingless bees shows convincing anti-inflammatory activity which is partly mediated through the regulation of the expression of inflammation-associated cytokines.

}, keywords = {Anti-inflammatory, Honey, IL-1β, IL-6, Philippine stingless bees, TNF-α.}, doi = {10.5530/pres.15.2.028}, author = {Mark Joseph Maranan Desamero and Mikaela Bernadette Andres Rabino and Maria Angelica Rimas Tayao and Mary Jasmin Cabillon Ang and Jussiaea Valente Bariuan and Roxanne Posilero Gapasin and Therese Marie Avena Collantes and Melissa Marie Risos Rondina and Cherry Pambid Fernandez-Colorado and Cleofas Rodriguez Cervancia and Maria Amelita Celino Estacio} } @article {1072, title = {Anti-inflammatory Potential of Euphorbia helioscopia Extracts against RAW264.7 Macrophages}, journal = {Pharmacognosy Research}, volume = {14}, year = {2022}, month = {July 2022}, pages = {338-341}, type = {Original Article}, chapter = {338}, abstract = {

Background: Euphorbia helioscopia, commonly known as sun spurge, is one of the largest species of an annual herb in the family Euphorbiaceae, which is extensively distributed worldwide. In folk medicine, it is used to treat various ailments such as malaria, osteomyelitis, and gastrointestinal ailments. The anti-inflammatory potential of Euphorbia helioscopia extracts on LPS-stimulated RAW264.7 macrophages was investigated in this work. Results: The ethanol extract was found to markedly inhibit the pro-inflammatory cytokines (IL-6 and TNFα) production compared to the aqueous extract treated cells. Moreover, extracts downregulated the nitric oxide levels. Finally, in this study, we have shown that Euphorbia helioscopia exerts a potent pharmacological effect on reducing the inflammatory response in macrophages. Conclusion: The results of the experiment revealed that the Euphorbia helioscopia extracts used in this study have anti-inflammatory characteristics, with the ethanolic extract having the most. The ethanolic extract had the strongest anti-inflammatory impact in terms of greatest reduction of proinflammatory indicators including IL-6, TNF-, and NO.

}, keywords = {Anti-inflammatory, ELISA, Euphorbia helioscopia, IL-6, TNF-α}, doi = {10.5530/pres.14.3.50}, author = {Iram Jan and Nusrit Iqbal Andrabi and Yassir Arfath and Tazeem Akram and Tusharika Kotra and Zabeer Ahmed and Mithilesh Kumar} }