@article {1422, title = {FTIR Analysis, Total Phenolic Content, Antioxidant and Antidiabetic Activities of Hildegardia populifolia (Roxb.) Schott and Endl. (Malvaceae)}, journal = {Pharmacognosy Research}, volume = {17}, year = {2025}, month = {April 2025}, pages = {679-688}, type = {Original Article}, chapter = {679}, abstract = {

Background: Diabetes has become a major public health issue in developing countries, with rising prevalence in urban populations. Type II diabetes, the most common form, can be managed with medication, diet and exercise. In India, Ayurvedic medicine utilizes botanicals for treating various conditions. Hildegardia populifolia, an endangered plant, has shown promise due to its therapeutic properties. Objectives: The aim and objectives of this study were to identify the active constituents by preliminary phytochemical screening, characterise the functional groups using Fourier Transform Infrared Spectrosocpy, analyse the total amount of phenols and to study the antioxidant and antidiabetic activities of aqueous leaf extract of Hildegardia populifolia. Materials and Methods: Total phenolic content was measured with the Folin-Ciocalteu reagent and antioxidant activity was determined using DPPH free radical scavenging assay. Anti-diabetic potential was assessed by 3,5-dinitrosalicylic acid method using α-amylase inhibition assay. Results: Phytochemical study of the extract revealed the presence of phenols, flavonoids, glycosides, tannins and terpenoids. The total phenolic content was 27.05 mg Gallic Acid Equivalent (GAE) per gram of extract. FTIR analysis showed the presence of 16 peaks representing functional groups like OH, C=C, C=O, C-Br, etc. The plant extract demonstrated good antioxidant and anti-diabetic activities, likely to the standard, ascorbic acid and acarbose respectively. Conclusion: These findings suggest that Hildegardia populifolia has strong potential for future research in developing therapeutic agents with both antioxidant and anti-diabetic properties, contributing to the search for innovative treatments for diabetes.

}, keywords = {3, 5-dinitrosalicylic acid, Acarabose, Folin-Ciocalteu, α-Amylase}, doi = {10.5530/pres.20252113}, author = {Satyavarapu Veera Venkata Naga Satya Mahalakshmi and Durga Bhavani Penmetsa and Kondal Reddy Yennam and Sadasivaiah Byalla} } @article {1020, title = {Isolation, Simultaneous Quantification of Taxifolin and Taxifolin-3-O-rhamnoside and Validation by RPHPLC}, journal = {Pharmacognosy Research}, volume = {14}, year = {2022}, month = {December 2021}, pages = {30-34}, type = {Original Article}, chapter = {30}, abstract = {

Background: Taxifolin (TA) is a flavonoid that has antioxidant, hepatoprotective, antialzheimer, anti-hyperglycemic, cardiovascular, anti-inflammatory, anti-psoriatic and antialzheimer properties. Taxifolin 3-O-rhamnoside (TAR), a glycoside of taxifolin, has antioxidant, anticonvulsant, anticancer, anti-inflammatory, and immunosuppressive effects. Objectives: The goal of this study is to separate TA and TAR from Smilax china Linn. rhizomes, as well as to develop and validate a technique for simultaneous measurement of TA and TAR. Materials and Methods: The hydro alcoholic extract of the rhizome of S. china yielded TA and TAR. HPLC system was fitted with a C18 column (shim-pack) of size 150 mm x 4.6 mm; 5μ, with suitable eluting mixture of methanol: water (90:10 v/v) at a flowing rate of 1 ml/min and at 254 nm wavelength for identification of peaks. Lab solution software was used to establish the quantification method for above-mentioned chemical compounds. Results: TAR and TA were separated using the proposed technique at Rt 2.917 and 3.924 min respectively. Over the range of 0.1-0.8 μg/ml, calibration curves were produced with a linear relationship R2 \> 0.9941 and 0.9963, respectively. The relative standard deviation was less than 2\%. The percentage recoveries were shown to be between 97 and 102.1. TA and TAR had detection limits of 0.156 and 0.077 μg/ml; quantification limits were 0.473 and 0.234 μg/ml respectively. The technique that was devised was simple, sensitive and specific. Conclusion: The newly designed RPHPLC technique has improved specificity, precision, and accuracy. The quality of S. china and other dietary supplements containing these two flavonoids may be successfully assessed by quantifying these two flavonoids and their glycosides.

}, keywords = {3, 3{\textquoteright}, 4{\textquoteright}-pentahydroxy flavanone, 5, 7, Astilbin, Dihydroqucertin, Tuberculosis, Validation.}, doi = {10.5530/pres.14.1.6}, url = {https://phcogres.com/fulltext/v14i1/105530pres1416.html}, author = {Subashini Subramanian and Shakila Ramachandran} }