@article {995, title = {GC-MS analysis of Phytocomponents in the Methanol Extract of Premna latifolia Roxb}, journal = {Pharmacognosy Research}, volume = {14}, year = {2022}, month = {December 2021}, pages = {19-23}, type = {Original Article}, chapter = {19}, abstract = {

Background: According to existing literature, the genus Premna has a variety of biologically active secondary metabolites, but there are few findings on phytochemical screening of P. latifolia Roxb. Methanolic leaves extract. Objectives: Phytochemical screening of methanolic leaves extract of P. latifolia was proposed for this study. Materials and Methods: UV-chamber, HPTLC instrument (CAMAG TLC Scanner), and GC-MS instrument (Perkin-Elmer GC- Clarus) were used to analyse methanolic leaves extract of P. latifolia. To compare the peaks of components on chromatograms, the NIST library was employed. Results: The current study used GC-MS to identify probable chemical components of P. latifolia. The GC-MS analysis and NIST library comparison revealed that the methanolic extract of P. latifolia contained mainly Squalene (13.57 percent), Ergosta-5, 7, 9 (11), 22-tetraen-3-ol, (3. beta, 22E)-(0.15 percent), Stigmasterol (3.73 percent), gamma-Sitosterol (10.13 percent), Lupeol (0.33), beta-Amyrin (2.27 percent), alpha-Amyrin (2.05 percent), gamma-Sitostenone (0.35 percent), Ursolic aldehyde (1.01 percent) and Betulin (0.72 percent). The biological actions of the majority of the identified components have been reported. While scientific evidence of gamma-sitostenone{\textquoteright}s biological function is still not available. Conclusion: According to the findings, P. latifolia contains a variety of biologically active components, the majority of which are tri-terpenoids and phytosterols.

}, keywords = {GC-MS Analysis, HPTLC, Methanolic leaves extract., Phytocomponents TLC, Premna latifolia}, doi = {10.5530/pres.14.1.4}, url = {https://phcogres.com/fulltext/v14i1/105530pres1414.html}, author = {Rajesh Kumar and Brijesh Kumar and Ashutosh Kumar and Ajay Kumar and Manish Singh} }