@article {109, title = {Chemical Composition and Antioxidant Activity of Origanum elongatum Essential Oil}, journal = {Pharmacognosy Research}, volume = {11}, year = {2019}, month = {August 2019}, pages = {283-289}, type = {Original Article}, chapter = {283}, abstract = {

Background: In recent years, antioxidants have become essential in nutrition because of their ability to protect the body against oxidative cell damage that can produce dangerous diseases. Medicinal plants are a major source of many bioactive molecules that act directly on the body. Objective: The main objective of our work is to highlight the antioxidant potential of the essential oil (EO) of Origanum elongatum Emb and Maire (endemic plant of Morocco), determine its composition, and carry out fractionation operations to isolate the molecule (s) responsible for this activity. Materials and Methods: The measurement of the antioxidant activity of the EO is carried out by trapping the free radical 2,2-diphenyl-1-picrylhydrazyl and the composition of the EO is determined using gas chromatography coupled with mass spectrometry. The fractionation of the EO was carried out by various chromatography techniques. Results: The results show that the EO has an interesting antioxidant activity with an EC50 in the order of 1.2 in comparison with a reference antioxidant, δ-tocopherol whose EC50 is 0.26. The EO of O. elongatum consists of 11 compounds, of which 3 are the majority: carvacrol (60.42\%), para-cymene (13.9\%), and γ-terpinene (9.4\%). Conclusion: Fractionation of the oil made it possible to isolate a purified antioxidant molecule consisting mainly of carvacrol (95\%).

}, keywords = {2, 2-diphenyl-1-picrylhydrazyl, Antioxidant activity, Essential oil composition, Fractionation, Origanum elongatum.}, doi = {10.4103/pr.pr_157_18}, author = {Hanane Oualili and Rachid Nmila and Fatima Chibi, Mariama Lasky and Abderrahmane Mricha and Halima Rchid} } @article {618, title = {Volatile Constituents of Pinus Roxburghii from Nepal}, journal = {Pharmacognosy Research}, volume = {5}, year = {2013}, month = {January 2013}, pages = {43-48}, type = { Original Article}, chapter = {43}, abstract = {

Background:Pinus roxburghii\ Sarg. Is one of 3 species of pine found in Nepal, the oil of which is traditionally used to treat cuts, wounds, boils, and blisters.\ Objective:\ To obtain, analyze, and examine the anti-microbial and cytotoxic activities of the essential oils of\ P. roxburghii.\ Materials and Methods:\ Three plant parts (cone, needle, and bark) of\ Pinus roxburghii\ were collected in Biratnagar, Nepal. The essential oils were obtained by hydrodistillation, and the chemical compositions were determined by GC-MS. The needle and cone essential oils were screened for anti-microbial activity against\ Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa,Escherichia coli, and\ Aspergillus niger;\ brine shrimp (Artemia salina) lethality; and\ in-vitro\ cytotoxicity against MCF-7 cells.\ Results:\ GC-MS analysis for the cone oil revealed 81 compounds with 78 components being identified (95.5\% of the oil) while 98.3\% of needle oil was identified to contain 68 components and 98.6\% of the bark oil (38 components) was identified. The 3 essential oils were dominated by sesquiterpenes, particularly (E)-caryophyllene (26.8\%-34.5\%) and α-humulene (5.0\%-7.3\%) as well as monoterpene alcohols terpinen-4-ol (4.1\%-30.1\%) and α-terpineol(2.8\%-5.0\%). The monoterpene δ-3-carene was present only in needle and cone essential oils (2.3\% and 6.8\%, respectively). Bio-activity assays of the cone essential oil of\ P. roxburghii\ showed remarkable cytotoxic activity (100\% killing of MCF-7 cells at 100 μg/mL) along with notable brine shrimp lethality (LC50\ =11.8 μg/mL). The cone essential oil did not show anti-bacterial activity, but it did exhibit anti-fungal activity against\ Aspergillus niger\ (MIC=39 μg/mL).\ Conclusion:\ The bioactivity of\ P. roxburghii\ essential oil is consistent with its traditional medicinal use.

}, keywords = {Anti-fungal, Brine shrimp lethality, cytotoxicity, Essential oil composition, α-humulene}, doi = {10.4103/0974-8490.105650}, author = {Prabodh Satyal and Prajwal Paudel and Josna Raut and Akash Deo and Noura S Dosoky and William N Setzer} } @article {808, title = {Essential Oil Composition of the Dracocephalum moldavica L from Xinjiang in China}, journal = {Pharmacognosy Research}, volume = {1}, year = {2009}, month = {January 2010 }, pages = {172-174}, type = {Original Article}, chapter = {172}, abstract = {

The essential oil of Dracocephalum moldavica L from Xinjiang in China was isolated by hydrodistillation in yield of 0.15 \%(w/w). The chemical composition of the essential oil was analyzed by GC and GCMS. Fifty-one compounds accounting for 99.45\% of the total oil were identified. The major components wereα-Citral (32.55\%),β-Citral (23.53\%), Acetic acid, geranial ester(21.32\%), Trans-Geraniol(3.38\%), Nerolacetate(3.38\%), Octane(2.14\%), and 2,4,6-Trimethyl -3-cyclohexene-1-carboxaldehyde (1.3\%). Monotenepers were the main group of compounds.

}, keywords = {Dracocephalum moldavica L, Essential oil composition, GC-MS}, doi = {Nil}, author = {Tian Shuge and Zhou Xiaoying and Zhang Fan and An Dongqing and Yang Tao} }