@article {1316, title = {Evaluation of Fractions of Artocarpus lakoocha R. on Doxorubicin Induced Myocardial Toxicity in Wistar Rats}, journal = {Pharmacognosy Research}, volume = {16}, year = {2024}, month = {October 2024}, pages = {897-907}, type = {Original Article}, chapter = {897}, abstract = {

Background: Doxorubicin, an anthracycline derivative, is a cytotoxic drug that has been shown to be effective against a variety of cancers. Its clinical utility has been limited due to dose-dependent cardiac toxicity. Objectives: To fractionate the ethanolic extract of bioactive Artocarpus lakoocha Roxb. bark and to Phytochemical investigation of fractions and pharmacological evaluation on doxorubicin induced cardiotoxicity in rats. Materials and Methods: In vitro studies conducted to determine Cardioprotective activity of the fractions of ethanolic plant extract. Cardiotoxicity induced with DOX (2.5 mg/kg body weight i.p) in 6 equal injections given alternatively for 2 weeks to make a total cumulative dose of 15 mg/kg body weight. Comparative studies were done between Treatment group, Dox 2.5 mg/kg on alternative day for 2 weeks followed by fractionation method-Petroleum ether fraction (11.2 mg/kg), Chloroform fraction (66 mg/kg), Ethyl acetate fraction (34 mg/kg) and aqueous fraction (44.4 mg/kg) of Artocarpus lakoocha R. for 2 weeks. Animals were observed for change in body weight, food and water intake ECG changes, hematology analysis such as RCB, WBC, Platelet, hematocrite level, Cardiac markers such as CK-MB, LDH and cTn I, Antioxidant enzymes such as GSH, Catalases, SOD and MDA were monitored. Heart histopathological studies carried out to evaluate cardiotoxicity. Results: As a result of Dox treatment, cardiomyopathy develops, which is characterized by an increase in cardiac biomarkers, changes in ECG, hematology analysis and a deficiency in antioxidant enzymes. By lowering the elevated levels of biomarker enzymes like LDH and CK-MB and the absence of CTnl, Fractions of EAAL significantly protected the myocardium from the toxic effects of Dox. Conclusion: Based on results of general appearance, hematology analysis, ECG studies, specific cardiac markers and antioxidant activity, it is concluded that the ethyl acetate fraction of Artocarpus lakoocha R. exhibited cardioprotective activity. Based on data obtained, treatment studies were found more effective when compared with preventive studies. Phytocompounds present in the plant is responsible for the cardioprotective activity.

}, keywords = {Artocarpus lakoocha R., cardiotoxicity, Doxorubicin, Oxidative stress, Rats}, doi = {10.5530/pres.16.4.102}, author = {Shreya Nagmoti and Shrishail K. Nimbal and Hasanpasha N. Sholapur and Navya S and Siddalingaswamy Hiremath} } @article {950, title = {Combined effects of p-coumaric acid and naringenin against doxorubicin-induced cardiotoxicity in rats}, journal = {Pharmacognosy Research}, volume = {3}, year = {2011}, month = {September 2011}, pages = {214-219}, type = {Original Article}, chapter = {214}, abstract = {

Background: Doxorubicin (DOX) is the most active cytotoxic agents having efficacy in malignancies either alone or combined with other cytocidal agents. The clinical usefulness of the anthracycline drug has been precluded by cardiac toxicity. Many therapeutic interventions have been attempted to improve the therapeutic benefits of the drug. This study is based on the possible protective effects of combination of p-coumaric acid (PC) and naringenin (NR) on DOX induced cardiac toxicity in male Swiss albino rats. Methods: Total nine groups of Swiss albino rats were used, Group I (vehicle control) receive saline solution daily and Group II (disease control) receive saline solution daily up to 29 th day and at 30 th day a single dose of DOX (15 mg/kg i.p.) is given. PC alone (100 mg/kg/day p.o.) and (200 mg/kg/day p.o.) also NR alone (15 mg/kg/day) orally administer for 30 days. Similarly a standard drug Vit. E (100 mg/kg/day) administers alone for 30 days. Group PC/DOX and PC and NR/DOX receive PC (200 mg/kg/day) and combine PC (200 mg/kg/day). Results: Doxorubicin induced marked biochemical alterations characteristic of cardiac toxicity including increase in MDA level and decrease SOD, CAT \& GSH level but prior administration of combination of PC \& NR ahead of doxorubicin challenge ameliorated all these biochemical markers. Conclusion: The study proves the beneficial effects of combination of PC and NR in protecting animal against DOX induced cardiotoxicity.

}, keywords = {Antioxidents, cardiotoxicity, doxorubicine, naringenin, p-coumaric acid}, doi = {10.4103/0974-8490.85012}, author = {Shruti S Shiromwar and Vijay R Chidrawar} }