@article {1281, title = {Screening of in vitro Anticancer Activity of Various Human Cancer Cell Lines and Induced Apoptosis and Cell Arrest by Ethanolic Bulb Extract of Pancratium zeylanicum}, journal = {Pharmacognosy Research}, volume = {16}, year = {2024}, month = {October 2024}, pages = {718-726}, type = {Original Article}, chapter = {1}, abstract = {

Aim: World widely the human society suffers from various types of cancer due to many causes. Thus, finding out a good anticancer agent is a need of the hour. Medicinal herbs are always considered at pole position whenever we discuss anticancer properties. Numerous herbal plants have been assessed and are subjected to phytochemical analysis to learn more about their anticancer effects. Materials and Methods: To assess the anti-cancerous activity of PZBE extract in MTT assay, cell cycle analysis, and apoptosis studies have been performed using different cancer cell lines. Results: This study aims to assess the impact of ethanolic extract of Pancratium zeylanicum Bulbs (PZBE) against cancerous cells in vitro conditions. MTT assay was used to compare the PZBE extract with Annexin V as a positive control compound against four human cancer cell lines, including Human cervical cancer (HeLa), colon cancer (HT-29), lung cancer (A549) and breast cancer (MCF-7) cell lines with the IC50 values of 209.28 μg/mL for HeLa, 133.09 μg/mL for A-549, 264.46 μg/mL for MCF-7 and 264.75 μg/mL for HT-29 cell lines. Increasing of the populations observed in G2-M and G0-G1 cells by 42\% and 44.78\%, respectively when compared to the control. The graph demonstrates that PZBE extracts significantly induced G0-G1 arrest in A-549 cells. Furthermore, the PZBE extract effectively induced apoptosis when compared to untreated cells, A-549 cells treated with IC50 concentration, and annexin-V double-staining assay demonstrated an increase in the percentage of early and late apoptotic cells. Conclusion: The ethanol extract of Pancratium zeylanicum bulbs demonstrated potent cytotoxic activity against the cancer cell lines HeLa, A-549, MCF-7, and HT-29. The current study{\textquoteright}s findings may be helpful in the development of plant-based anticancer drugs.

}, keywords = {A-549, Annexin-V, Anticancer activity, Apoptosis, Ethanol extract, HeLa, HT-29, MCF-7}, doi = {10.5530/pres.16.4.83}, author = {Elumalai Shanthi Priya and Devaraj Sathiya Sheela and Devaraj Sheeba Gnanadeebam and Balrajmani Davidson Raja and Periannan Viswanathan} } @article {801, title = {Antiproliferative Activity and Induction of Apoptosis in Estrogen Receptor-positive and Negative Human Breast Carcinoma Cell lines by Gmelina asiatica Roots}, journal = {Pharmacognosy Research}, volume = {2}, year = {2010}, month = {May 2010}, pages = {113-119}, type = {Original Article}, chapter = {113}, abstract = {

Low risk of breast cancer has been proposed to be associated with high intake of lignans. We have reported the presence of lignans in Gmelina asiatica roots. There are no scientific reports on the antiproliferative activity of G. asiatica roots. The objective of the present study was to evaluate the effect of ethyl acetate extract from G. asiatica roots (EGAR) on estrogen receptor-positive (MCF-7) and negative (MDA-MB-231) human breast cancer cell lines. The effects of 50\% inhibitory concentrations (IC50) of EGAR on MCF-7 and MDA-MB-231 cells were determined using 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay kit. The mode of cell death caused by EGAR was determined using dual apoptosis assay kit by observing the cells under fluorescent microscope. The quantification of apoptosis and necrosis in cells caused by EGAR was determined using cell death detection kit through ELISA. Down-regulation of the proliferative activity occurred in a clear dose-dependent response with IC50 values of 32.9 {\textpm} 3.8 μg/mL in MCF-7 and 19.9 {\textpm} 2.3 μg/ mL in MDA-MB-231 cell lines. Treatment of breast cancer cells with EGAR resulted in significant apoptosis. The EGAR contain lignans and flavonoids. The antiproliferative activity of the extract is attributed to the presence of these secondary metabolites. The results suggest the efficacy of G. asiatica roots as antiproliferative agents on human breast cancer cells, supporting the hypothesis that plants containing lignans have beneficial effects on human breast cancer.

}, keywords = {Annexin-V, Caspase-3, Enrichment factor, Lignans, MCF-7, MDA-MB-231, Phosphatidylserine}, doi = {10.4103/0974-8490.62949}, author = {Madhu Katyayani Balijepalli and Satyanarayana Tandra and Mallikarjuna Rao Pichika} }